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PMID: 2030975 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Rapid and simple purification of T7 RNA polymerase.

Nucleic acids research ·Vol. 19 ·No. 8 ·1991-04-25 ·Pages 1948

Zawadzki V, Gross HJ

Abstract

暂无摘要

MeSH Terms
DNA-Directed RNA Polymerases/isolation & purification Electrophoresis, Polyacrylamide Gel Methods T-Phages/enzymology
Chemicals
DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zawadzki V
Institut für Biochemie, Bayerische Julius-Maximilians-Universität, Würzburg, FRG.
Gross H J
References (5)
5 references, click to expand
  1. On the use of T7 RNA polymerase transcripts for physical investigation.
    Nucleic Acids Res. 1990 Jul 25;18(14):4139-42 PMID: 1696001
  2. Cloning and expression of the gene for bacteriophage T7 RNA polymerase.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):2035-9 PMID: 6371808
  3. Synthesis of small RNAs using T7 RNA polymerase.
    Methods Enzymol. 1989;180:51-62 PMID: 2482430
  4. ompT encodes the Escherichia coli outer membrane protease that cleaves T7 RNA polymerase during purification.
    J Bacteriol. 1988 Mar;170(3):1245-53 PMID: 3277950
  5. Biochemical and physical characterization of an unmodified yeast phenylalanine transfer RNA transcribed in vitro.
    Proc Natl Acad Sci U S A. 1988 Feb;85(4):1033-7 PMID: 3277187
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
Published
1991-04-25
Pages
1948
Language
English
Region
England
NLM ID
0411011
PMCID
PMC328134
Subset
IM
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