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PMID: 2030941 Published · ppublish English Journal Article

CAAT/enhancer binding protein is able to bind to ATF/CRE elements.

Nucleic acids research ·Vol. 19 ·No. 6 ·1991-03-25 ·Pages 1213-7

Bakker O, Parker MG

Abstract

The transcription factor C/EBP is known to be able to bind to two different classes of sites, a CAAT-box and one present in a number of viral enhancers. In this paper we show using band-shift assays, methylation interference and footprinting that C/EBP is also able to bind with high affinity to ATF/CRE sites. Competition with mutant ATF sites and methylation interference indicate that C/EBP may be able to bind to the ATF/CRE sites by virtue of their homology to the enhancer core elements. Furthermore, we show that C/EBP is able to direct transcription from this site in transient transfection experiments and that mutations in the ATF binding site that impair DNA binding also effect the ability of C/EBP to stimulate transcription.

MeSH Terms
Activating Transcription Factors Animals Base Sequence Binding, Competitive Blood Proteins/metabolism CCAAT-Enhancer-Binding Proteins DNA Fingerprinting DNA-Binding Proteins/metabolism Enhancer Elements, Genetic HeLa Cells Humans Male Methylation Molecular Sequence Data Nuclear Proteins/metabolism Plasmids Rats Rats, Inbred Strains Transcription Factors/metabolism Transcription, Genetic Transfection
Chemicals
Activating Transcription Factors Blood Proteins CCAAT-Enhancer-Binding Proteins DNA-Binding Proteins Nuclear Proteins Transcription Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bakker O
Molecular Endocrinology Laboratory, Imperial Cancer Research Fund, London, UK.
Parker M G
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.6.1213
Published
1991-03-25
Pages
1213-7
Language
English
Region
England
NLM ID
0411011
PMCID
PMC333845
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/19.6.1213 PMC Full Text View journal details
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