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题目:: Manipulating CD4+ T cells by optical tweezers for the initiation of cell-cell transfer of HIV-1.
作者:: McNerney(Gregory P),Hübner(Wolfgang),Chen(Benjamin K),Huser(Thomas)
状态:: 发布时间2010-04-07 , 更新时间 2016-11-22
期刊:: J Biophotonics
摘要:: Cell-cell interactions through direct contact are very important for cellular communication and coordination - especially for immune cells. The human immunodeficiency virus type I (HIV-1) induces immune cell interactions between CD4(+) cells to shuttle between T cells via a virological synapse. A goal to understand the process of cell-cell transmission through virological synapses is to determine the cellular states that allow a chance encounter between cells to become a stable cell-cell adhesion. We demonstrate the use of optical tweezers to manipulate uninfected primary CD4(+) T cells near HIV Gag-iGFP transfected Jurkat T cells to probe the determinants that induce stable adhesion. When combined with fast 4D confocal fluorescence microscopy, optical tweezers can be utilized not only to facilitate cell-cell contact, but also to simultaneously track the formation of a virological synapse, and ultimately to probe the events that precede virus transfer.
语言:: eng
DOI:

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