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PMID: 202962 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Interspecies radioimmunoassay for the major structural proteins of primate type-D retroviruses.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 12 ·1977-12-00 ·Pages 5739-43

Colcher D, Teramoto YA, Schlom J

Abstract

A competition radioimmunoassay has been developed in which type-D retroviruses from three primate species compete. The assay utilizes the major structural protein (36,000 daltons) of the endogenous squirrel monkey retrovirus and antisera directed against the major structural protein (27,000 daltons) of the Mason-Pfizer monkey virus isolated from rhesus monkeys. Purified preparations of both viruses grown in heterologous cells, as well as extracts of heterologous cells infected with squirrel monkey retrovirus or Mason-Pfizer monkey virus, compete completely in the assay. Addition of an endogenous virus of the langur monkey also results in complete blocking. No blocking in the assay is observed with type-C baboon viruses, woolly monkey virus, and gibbon virus. Various other type-C and type-B viruses also showed no reactivity. An interspecies assay has thus been developed that recognizes the type-D retroviruses from both Old World monkey (rhesus and langur) and New World monkey (squirrel) species.

MeSH Terms
Animals Binding, Competitive Cell Line Glycoproteins/immunology Haplorhini/microbiology Molecular Weight Radioimmunoassay/methods Retroviridae/immunology Species Specificity Viral Proteins/analysis,immunology
Chemicals
Glycoproteins Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Colcher D
Teramoto Y A
Schlom J
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.12.5739
Published
1977-12-00
Pages
5739-43
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431869
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.12.5739 PMC Full Text View journal details
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