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PMID: 2023934 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Anticodon-dependent aminoacylation of a noncognate tRNA with isoleucine, valine, and phenylalanine in vivo.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 9 ·1991-05-01 ·Pages 3872-6

Pallanck L, Schulman LH

Abstract

An assay based on the initiation of protein synthesis in Escherichia coli has been used to explore the role of the anticodon in tRNA identity in vivo. Mutations were introduced into the initiator tRNA to change the wild-type anticodon from CAU (methionine) to GAU (isoleucine), GAC (valine), and GAA (phenylalanine), where each derivative differs from the preceding by a single base change in the anticodon (underlined). These changes were sufficient to cause the mutant tRNAs to be aminoacylated by the corresponding aminoacyl-tRNA synthetases based on the amino acid inserted into protein from complementary initiation codons. Construction of additional single base anticodon variants (GUU, GGU, GCC, GUC, GCA, and UAA) showed that all three anticodon bases specify isoleucine and phenylalanine identity and that both the middle and the third anticodon bases are important for valine identity in vivo.

MeSH Terms
Amino Acyl-tRNA Synthetases/metabolism Anticodon Base Sequence Escherichia coli/genetics In Vitro Techniques Isoleucine/metabolism Isoleucine-tRNA Ligase/metabolism Molecular Sequence Data Peptide Chain Initiation, Translational Phenylalanine/metabolism Phenylalanine-tRNA Ligase/metabolism RNA, Transfer, Met/metabolism Substrate Specificity Valine/metabolism Valine-tRNA Ligase/metabolism
Chemicals
Anticodon RNA, Transfer, Met Isoleucine Phenylalanine Amino Acyl-tRNA Synthetases Phenylalanine-tRNA Ligase Isoleucine-tRNA Ligase Valine-tRNA Ligase Valine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pallanck L
Department of Developmental Biology and Cancer, Albert Einstein College of Medicine, Bronx, NY 10461.
Schulman L H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.9.3872
Published
1991-05-01
Pages
3872-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51555
Subset
IM
Grants
NCI NIH HHS · P30CA 13330 · United States
NIGMS NIH HHS · T32 GM07491 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.9.3872 PMC Full Text View journal details
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