Home LiteratureArticle Details

PMID: 202356 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The mechanism of action of narcotic analgesics in the guinea-pig ileum.

British journal of pharmacology ·Vol. 61 ·No. 4 ·1977-12-00 ·Pages 541-9

North RA, Tonini M

Abstract

1. Intracellular recordings were made from neurones in the myenteric plexus of the guinea-pig ileum. Single myenteric ganglia were maintained in vitro and drugs were applied by adding them to the perfusing solution. 2. Narcotic analgesics hyperpolarized the membrane of a proportion of neurones in the myenteric plexus. 3. The membrane hyperpolarization was sometimes associated with a decrease in input resistance. These effects reduced the excitability of myenteric neurones. 4. The effects of narcotics occurred at low concentrations (10 nM to 1 micrometer), were stereospecific and were reversed by naloxone. 5. It is proposed that the morphine-sensitive neurones may be the cholinergic efferents to the muscle layers. By hyperpolarizing these neurones, morphine may prevent their excitation by electric field stimulation. This may explain why narcotic analgesics reduce the output of acetylcholine and the contractile response of this preparation when it is excited by field stimulation.

MeSH Terms

Action Potentials/drug effects Animals Guinea Pigs Ileum/drug effects In Vitro Techniques Membrane Potentials/drug effects Morphine/antagonists & inhibitors,pharmacology Myenteric Plexus/drug effects Naloxone/pharmacology Neuromuscular Junction/drug effects Synaptic Transmission/drug effects

Chemicals

Naloxone Morphine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

North R A

Tonini M

References (24)

24 references, click to expand

Action of morphine on guinea-pig myenteric plexus and mouse vas deferens studied by intracellular recording.
Life Sci. 1975 Jul 1;17(1):63-6 PMID: 167255
Effect of synaptic transmission blockade on morphine action in the guinea-pig myenteric plexus.
J Pharmacol Exp Ther. 1976 Jan;196(1):97-106 PMID: 173830
In vitro models in the study of structure-activity relationships of narcotic analgesics.
Annu Rev Pharmacol. 1975;15:29-47 PMID: 238462
Receptor binding and pharmacological activity of opiates in the guinea-pig intestine.
J Pharmacol Exp Ther. 1975 Jul;194(1):205-19 PMID: 239219
(3H)morphine localization in myenteric plexus.
Science. 1976 Aug 20;193(4254):689-91 PMID: 948744
Effects of morphine on myenteric plexus neurones.
Neuropharmacology. 1976 Nov;15(11):719-21 PMID: 1004695
Single neuron studies of opiate action in the guinea pig myenteric plexus.
Life Sci. 1975 Jul 1;17(1):57-62 PMID: 1143011
Pharmacological properties of electrical activities obtained from neurons in Auerbach's plexus.
Jpn J Pharmacol. 1973 Oct;23(5):665-71 PMID: 4148816
Fine structure of the myenteric plexus in the guinea-pig ileum.
J Anat. 1972 Jan;111(Pt 1):69-97 PMID: 4335909
The contribution of membrane hyperpolarization to adaptation and conduction block in sensory neurones of the leech.
J Physiol. 1973 May;230(3):509-34 PMID: 4717151
Intracellular recording from the myenteric plexus of the guinea-pig ileum.
J Physiol. 1973 Jun;231(3):471-91 PMID: 4783093
A nervous mechanism for descending inhibition in guinea-pig small intestine.
J Physiol. 1974 Apr;238(1):129-43 PMID: 4838801
Effects of narcotic opiates and serotonin on the electrical behavior of neurons in the guinea pig myenteric plexus.
Life Sci. 1974 Jun 1;14(11):2299-309 PMID: 4847817
Comparison of effect of morphine-like analgesics on transmurally stimulated guinea-pig ileum.
Br J Pharmacol. 1969 Oct;37(2):436-49 PMID: 5348430
The effects of hexamethonium, morphine and adrenaline on the output of acetylcholine from the myenteric plexus-longitudinal muscle preparation of the ileum.
Br J Pharmacol. 1970 Nov;40(3):553P-554P PMID: 5497815
Kinetic parameters of narcotic agonists and antagonists, with particular reference to N-allylnoroxymorphone (naloxone).
Br J Pharmacol Chemother. 1968 Jun;33(2):266-76 PMID: 5664147
The effect of analgesic drugs on the release of acetylcholine from electrically stimulated guinea-pig ileum.
Br J Pharmacol Chemother. 1966 May;27(1):81-92 PMID: 5961471
Agonist and antagonist actions of morphine-like drugs on the guinea-pig isolated ileum.
Br J Pharmacol Chemother. 1966 Sep;27(3):514-27 PMID: 5970903
Influence of atropine and morphine on the liberation of acetylcholine from the guinea pig's intestine.
Nature. 1956 Nov 17;178(4542):1121-2 PMID: 13387645
Inhibition by morphine of the release of acetylcholine from the intestine of the guinea-pig.
Br J Pharmacol Chemother. 1957 Mar;12(1):115-8 PMID: 13413162
The action of morphine and related substances on contraction and on acetylcholine output of coaxially stimulated guinea-pig ileum.
Br J Pharmacol Chemother. 1957 Mar;12(1):119-27 PMID: 13413163
STIMULANT ACTIONS OF VOLATILE ANAESTHETICS ON SMOOTH MUSCLE.
Br J Pharmacol Chemother. 1964 Apr;22:356-65 PMID: 14190470
THE INHIBITION OF AUTONOMIC NEURO-EFFECTOR TRANSMISSION BY MORPHINE-LIKE DRUGS AND ITS USE AS A SCREENING TEST FOR NARCOTIC ANALGESIC DRUGS .
Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1964 May 25;248:231-46 PMID: 14230237
Two types of neurones in the myenteric plexus of duodenum in the guinea-pig.
J Physiol. 1974 Jan;236(2):303-26 PMID: 16992436

Article Info

Journal

British journal of pharmacology

Abbr.

Br J Pharmacol

ISSN

0007-1188

DOI

10.1111/j.1476-5381.1977.tb07546.x

Published

1977-12-00

Pages

541-9

Language

English

Region

England

NLM ID

7502536

PMCID

PMC1668067

Subset

External Links

PubMed source DOI: 10.1111/j.1476-5381.1977.tb07546.x PMC Full Text View journal details

Analysis Services

The mechanism of action of narcotic analgesics in the guinea-pig ileum.

Contact

WeChat Official Account