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PMID: 2023245 Published · ppublish English Journal Article

Crystallization of the hybrid Bacillus (1-3,1-4)-beta-glucanase H(A16-M).

Journal of molecular biology ·Vol. 218 ·No. 4 ·1991-04-20 ·Pages 703-4

Keitel T, Granzin J, Simon O, Borriss R, Thomsen KK, Wessner H, Höhne W, Heinemann U

Abstract

The extremely thermostable hybrid Bacillus (1-3,1-4)-beta-glucanase H(A16-M) has been crystallized with polyethylene glycol by vapour diffusion. The single crystals diffract to a resolution of 2.2 A. The protein crystallizes in the orthorhombic space group P2(1)2(1)2(1) with a = 70.22(7) A, b = 72.56(1) A, c = 49.97(1) A, and has one molecule per asymmetric unit.

MeSH Terms
Bacillus/enzymology Glycoside Hydrolases/chemistry X-Ray Diffraction
Chemicals
Glycoside Hydrolases licheninase
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Keitel T
Institut für Kristallographie, Freie Universität Berlin.
Granzin J
Simon O
Borriss R
Thomsen K K
Wessner H
Höhne W
Heinemann U
Article Info
Journal
Journal of molecular biology
Abbr.
J Mol Biol
ISSN
0022-2836
Published
1991-04-20
Pages
703-4
Language
English
Region
England
NLM ID
2985088R
Subset
IM
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