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PMID: 20208028 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Impact of siderophore production by Pseudomonas syringae pv. syringae 22d/93 on epiphytic fitness and biocontrol activity against Pseudomonas syringae pv. glycinea 1a/96.

Applied and environmental microbiology ·Vol. 76 ·No. 9 ·2010-05-00 ·Pages 2704-11

Wensing A, Braun SD, Büttner P, Expert D, Völksch B, Ullrich MS, Weingart H

Abstract

The use of naturally occurring microbial antagonists to suppress plant diseases offers a favorable alternative to classical methods of plant protection. The soybean epiphyte Pseudomonas syringae pv. syringae strain 22d/93 shows great potential for controlling P. syringae pv. glycinea, the causal agent of bacterial blight of soybean. Its activity against P. syringae pv. glycinea is highly reproducible even in field trials, and the suppression mechanisms involved are of special interest. In this work we demonstrated that P. syringae pv. syringae 22d/93 produced a significantly larger amount of siderophores than the pathogen P. syringae pv. glycinea produced. While P. syringae pv. syringae 22d/93 and P. syringae pv. glycinea produce the same siderophores, achromobactin and pyoverdin, the regulation of siderophore biosynthesis in the former organism is very different from that in the latter organism. The epiphytic fitness of P. syringae pv. syringae 22d/93 mutants defective in siderophore biosynthesis was determined following spray inoculation of soybean leaves. The population size of the siderophore-negative mutant P. syringae pv. syringae strain 22d/93DeltaSid was 2 orders of magnitude lower than that of the wild type 10 days after inoculation. The growth deficiency was compensated for when wound inoculation was used, indicating the availability of iron in the presence of small lesions on the leaves. Our results suggest that siderophore production has an indirect effect on the biocontrol activity of P. syringae pv. syringae 22d/93. Although siderophore-defective mutants of P. syringae pv. syringae 22d/93 still suppressed development of bacterial blight caused by P. syringae pv. glycinea, siderophore production enhanced the epiphytic fitness and thus the competitiveness of the antagonist.

MeSH Terms
Antibiosis Citrates/biosynthesis Ketoglutaric Acids Oligopeptides/biosynthesis Pest Control, Biological Plant Diseases/microbiology Plant Leaves/microbiology Pseudomonas syringae/metabolism Siderophores/biosynthesis Soybeans/microbiology
Chemicals
Citrates Ketoglutaric Acids Oligopeptides Siderophores achromobactin pyoverdin
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Wensing Annette
School of Engineering and Science, Jacobs University Bremen, Bremen, Germany.
Braun Sascha D
Büttner Petra
Expert Dominique
Völksch Beate
Ullrich Matthias S
Weingart Helge
References (40)
40 references, click to expand
  1. Disruption of N-acyl homoserine lactone-mediated cell signaling and iron acquisition in epiphytic bacteria by leaf surface compounds.
    Appl Environ Microbiol. 2006 Dec;72(12):7678-86 PMID: 16997987
  2. Traits of fluorescent Pseudomonas spp. involved in suppression of plant root pathogens.
    Microbiol Rev. 1992 Dec;56(4):662-76 PMID: 1480114
  3. Iron and metal regulation in bacteria.
    Curr Opin Microbiol. 2001 Apr;4(2):172-7 PMID: 11282473
  4. Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria.
    J Bacteriol. 1990 Nov;172(11):6568-72 PMID: 2172217
  5. Analysis of achromobactin biosynthesis by Pseudomonas syringae pv. syringae B728a.
    J Bacteriol. 2009 Jul;191(14):4594-604 PMID: 19482931
  6. Universal chemical assay for the detection and determination of siderophores.
    Anal Biochem. 1987 Jan;160(1):47-56 PMID: 2952030
  7. The structure of the pyoverdin isolated from various Pseudomonas syringae pathovars.
    Z Naturforsch C. 2001 Sep-Oct;56(9-10):687-94 PMID: 11724369
  8. WITHHOLDING AND EXCHANGING IRON: Interactions Between Erwinia spp. and Their Plant Hosts.
    Annu Rev Phytopathol. 1999;37:307-334 PMID: 11701826
  9. Biological control of soil-borne pathogens by fluorescent pseudomonads.
    Nat Rev Microbiol. 2005 Apr;3(4):307-19 PMID: 15759041
  10. The Genomic Sequence of Pseudomonas fluorescens Pf-5: Insights Into Biological Control.
    Phytopathology. 2007 Feb;97(2):233-8 PMID: 18944380
  11. Salicylic acid, yersiniabactin, and pyoverdin production by the model phytopathogen Pseudomonas syringae pv. tomato DC3000: synthesis, regulation, and impact on tomato and Arabidopsis host plants.
    J Bacteriol. 2007 Oct;189(19):6773-86 PMID: 17660289
  12. Pseudomonas fluorescens and closely-related fluorescent pseudomonads as biocontrol agents of soil-borne phytopathogens.
    Lett Appl Microbiol. 2009 May;48(5):505-12 PMID: 19291210
  13. Environmental factors modulating antibiotic and siderophore biosynthesis by Pseudomonas fluorescens biocontrol strains.
    Appl Environ Microbiol. 1999 Jun;65(6):2429-38 PMID: 10347023
  14. 3-Methylarginine from Pseudomonas syringae pv. syringae 22d/93 suppresses the bacterial blight caused by its close relative Pseudomonas syringae pv. glycinea.
    Chembiochem. 2008 Aug 11;9(12):1913-20 PMID: 18655083
  15. Characterization of fluorescent and nonfluorescent peptide siderophores produced by Pseudomonas syringae strains and their potential use in strain identification.
    Appl Environ Microbiol. 2001 Apr;67(4):1718-27 PMID: 11282626
  16. OxyR and SoxRS regulation of fur.
    J Bacteriol. 1999 Aug;181(15):4639-43 PMID: 10419964
  17. A biological sensor for iron available to bacteria in their habitats on plant surfaces.
    Appl Environ Microbiol. 1994 Jun;60(6):1934-41 PMID: 16349283
  18. Development, registration and commercialization of microbial pesticides for plant protection.
    Int Microbiol. 2003 Dec;6(4):245-52 PMID: 12955583
  19. Biological Control of Pseudomonas syringae pv. glycinea by Epiphytic Bacteria under Field Conditions.
    Microb Ecol. 2001 Feb;41(2):132-139 PMID: 12032618
  20. Antagonistic Activities of Epiphytic Bacteria from Soybean Leaves against Pseudomonas syringae pv. glycinea in vitro and in planta
    Microb Ecol. 1997 Sep;34(2):118-24 PMID: 9230099
  21. Biocontrol ability of fluorescent pseudomonads genetically dissected: importance of positive feedback regulation.
    Curr Opin Biotechnol. 2000 Jun;11(3):290-7 PMID: 10851149
  22. Erwinia chrysanthemi requires a second iron transport route dependent of the siderophore achromobactin for extracellular growth and plant infection.
    Mol Microbiol. 2005 Jan;55(1):261-75 PMID: 15612933
  23. Stability, frequency and multiplicity of transposon insertions in the pyoverdine region in the chromosomes of different fluorescent pseudomonads.
    J Gen Microbiol. 1992 Jul;138(7):1337-43 PMID: 1324974
  24. The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000.
    Proc Natl Acad Sci U S A. 2003 Sep 2;100(18):10181-6 PMID: 12928499
  25. Detection and differentiation of microbial siderophores by isoelectric focusing and chrome azurol S overlay.
    Biometals. 1994 Oct;7(4):287-91 PMID: 7812113
  26. The siderophore pyoverdine of Pseudomonas syringae pv. tabaci 6605 is an intrinsic virulence factor in host tobacco infection.
    J Bacteriol. 2010 Jan;192(1):117-26 PMID: 19854904
  27. Relative importance of fluorescent siderophores and other factors in biological control of Gaeumannomyces graminis var. tritici by Pseudomonas fluorescens 2-79 and M4-80R.
    Appl Environ Microbiol. 1991 Nov;57(11):3270-7 PMID: 1838240
  28. Differential induction of systemic resistance in Arabidopsis by biocontrol bacteria.
    Mol Plant Microbe Interact. 1997 Aug;10(6):716-24 PMID: 9245833
  29. Characterization of pPT23B, the plasmid involved in syringolide production by Pseudomonas syringae pv. tomato PT23.
    Plasmid. 1994 May;31(3):275-87 PMID: 8058820
  30. Characterization of a tonB mutation in Erwinia chrysanthemi 3937: TonB(Ech) is a member of the enterobacterial TonB family.
    Microbiology. 2000 Aug;146 ( Pt 8):2051-8 PMID: 10931909
  31. Heterogeneity of iron bioavailability on plants assessed with a whole-cell GFP-based bacterial biosensor.
    Microbiology. 2000 Oct;146 ( Pt 10):2435-45 PMID: 11021920
  32. Two simple media for the demonstration of pyocyanin and fluorescin.
    J Lab Clin Med. 1954 Aug;44(2):301-7 PMID: 13184240
  33. Microbiology of the phyllosphere.
    Appl Environ Microbiol. 2003 Apr;69(4):1875-83 PMID: 12676659
  34. Characterization of Pyoverdin(pss), the Fluorescent Siderophore Produced by Pseudomonas syringae pv. syringae.
    Appl Environ Microbiol. 1987 May;53(5):928-34 PMID: 16347352
  35. Use of siderophores to type pseudomonads: the three Pseudomonas aeruginosa pyoverdine systems.
    Microbiology. 1997 Jan;143 ( Pt 1):35-43 PMID: 9025276
  36. Achromobactin, a new citrate siderophore of Erwinia chrysanthemi.
    Z Naturforsch C. 2000 May-Jun;55(5-6):328-32 PMID: 10928541
  37. Iron transport in Escherichia coli: all has not been said and done.
    Biometals. 2006 Apr;19(2):159-72 PMID: 16718601
  38. pBBR1MCS: a broad-host-range cloning vector.
    Biotechniques. 1994 May;16(5):800-2 PMID: 8068328
  39. Characterization of two epiphytic bacteria from soybean leaves with antagonistic activities against Pseudomonas syringae pv. glycinea.
    J Basic Microbiol. 1996;36(6):453-62 PMID: 8956493
  40. Involvement of Pyochelin and Pyoverdin in Suppression of Pythium-Induced Damping-Off of Tomato by Pseudomonas aeruginosa 7NSK2.
    Appl Environ Microbiol. 1996 Mar;62(3):865-71 PMID: 16535275
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
1098-5336
DOI
10.1128/AEM.02979-09
Published
2010-05-00
Epub
2010-00-05
Pages
2704-11
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC2863448
Subset
IM
External Links
PubMed source DOI: 10.1128/AEM.02979-09 PMC Full Text View journal details
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