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PMID: 20204454 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Genetic factors that influence moenomycin production in streptomycetes.

Journal of industrial microbiology & biotechnology ·Vol. 37 ·No. 6 ·2010-06-00 ·Pages 559-66

Makitrynskyy R, Rebets Y, Ostash B, Zaburannyi N, Rabyk M, Walker S, Fedorenko V

Abstract

Moenomycin, a natural phosphoglycolipid product that has a long history of use in animal nutrition, is currently considered an attractive starting point for the development of novel antibiotics. We recently reconstituted the biosynthesis of this natural product in a heterologous host, Streptomyces lividans TK24, but production levels were too low to be useful. We have examined several other streptomycetes strains as hosts and have also explored the overexpression of two pleiotropic regulatory genes, afsS and relA, on moenomycin production. A moenomycin-resistant derivative of S. albus J1074 was found to give the highest titers of moenomycin, and production was improved by overexpressing relA. Partial duplication of the moe cluster 1 in S. ghanaensis also increased average moenomycin production. The results reported here suggest that rational manipulation of global regulators combined with increased moe gene dosage could be a useful technique for improvement of moenomycin biosynthesis.

MeSH Terms
Animals Anti-Bacterial Agents/biosynthesis,isolation & purification Bambermycins/biosynthesis,isolation & purification Carbohydrate Sequence Cloning, Molecular Gene Dosage Gene Expression Regulation, Bacterial Genes, Regulator Molecular Sequence Data Streptomyces/genetics,metabolism Streptomyces lividans/genetics,metabolism
Chemicals
Anti-Bacterial Agents Bambermycins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Makitrynskyy Roman
Department of Genetics and Biotechnology, Ivan Franko National University of L'viv, Grushevskogo st. 4, L'viv 79005, Ukraine.
Rebets Yuriy
Ostash Bohdan
Zaburannyi Nestor
Rabyk Mariia
Walker Suzanne
Fedorenko Victor
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Article Info
Journal
Journal of industrial microbiology & biotechnology
Abbr.
J Ind Microbiol Biotechnol
ISSN
1476-5535
DOI
10.1007/s10295-010-0701-1
Published
2010-06-00
Epub
2010-00-06
Pages
559-66
Language
English
Region
Germany
NLM ID
9705544
PMCID
PMC2939378
Subset
IM
Grants
NIGMS NIH HHS · R01 GM076710-04 · United States
NIAID NIH HHS · R01 AI050855 · United States
NCRR NIH HHS · P41 RR015301-075426 · United States
NIAID NIH HHS · R01 AI050855-06 · United States
NIAID NIH HHS · AI50855 · United States
NIGMS NIH HHS · R01 GM076710 · United States
NIAID NIH HHS · P01 AI083214 · United States
NIAID NIH HHS · P01 AI083214-026668 · United States
External Links
PubMed source DOI: 10.1007/s10295-010-0701-1 PMC Full Text View journal details
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