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PMID: 20195578 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Review

Hydrogen exchange mass spectrometry: what is it and what can it tell us?

Analytical and bioanalytical chemistry ·Vol. 397 ·No. 3 ·2010-06-00 ·Pages 967-72

Marcsisin SR, Engen JR

Abstract

Proteins are undoubtedly some of the most essential molecules of life. While much is known about many proteins, some aspects still remain mysterious. One particularly important aspect of understanding proteins is determining how structure helps dictate function. Continued development and implementation of biophysical techniques that provide information about protein conformation and dynamics is essential. In this review, we discuss hydrogen exchange mass spectrometry and how this method can be used to learn about protein conformation and dynamics. The basic concepts of the method are described, the workflow illustrated, and a few examples of its application are provided.

MeSH Terms
Animals Humans Hydrogen/chemistry Mass Spectrometry/methods,trends Protein Conformation Proteins/chemistry,metabolism
Chemicals
Proteins Hydrogen
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Marcsisin Sean R
The Department of Chemistry & Chemical Biology and The Barnett Institute of Chemical & Biological Analysis, Northeastern University, Boston, MA 02115, USA.
Engen John R
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Article Info
Journal
Analytical and bioanalytical chemistry
Abbr.
Anal Bioanal Chem
ISSN
1618-2650
DOI
10.1007/s00216-010-3556-4
Published
2010-06-00
Epub
2010-00-01
Pages
967-72
Language
English
Region
Germany
NLM ID
101134327
PMCID
PMC2868954
Subset
IM
Grants
NIGMS NIH HHS · R01 GM070590 · United States
NIGMS NIH HHS · R01 GM086507-01 · United States
NIGMS NIH HHS · R01 GM086507 · United States
NIGMS NIH HHS · R01 GM070590-06 · United States
NIGMS NIH HHS · GM070590 · United States
NIGMS NIH HHS · R01-GM086507 · United States
External Links
PubMed source DOI: 10.1007/s00216-010-3556-4 PMC Full Text View journal details
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