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题目:: Vacuum-assisted cell seeding in a microwell cell culture system.
作者:: Ferrell(Nicholas),Gallego-Perez(Daniel),Higuita-Castro(Natalia),Butler(Randall T),Reen(Rashmeet K),Gooch(Keith J),Hansford(Derek J)
状态:: 发布时间2010-03-12 , 更新时间 2010-03-12
期刊:: Anal Chem
摘要:: We present a simple method to actively pattern individual cells and groups of cells in a polymer-based microdevice using vacuum-assisted cell seeding. Soft lithography is used to mold polymer microwells with various geometries on top of commercially available porous membranes. Cell suspensions are placed in a vacuum filtration setup to pull culture medium through the microdevice, trapping the cells in the microwells. The process is evaluated by determining the number of cells per microwell for a given cell seeding density and microwell geometry. This method is tested with adherent and nonadherent cells (NIH 3T3 fibroblasts, PANC-1 pancreatic ductal epithelial-like cells, and THP-1 monocytic leukemia cells). These devices could find applications in high-throughput cell screening, cell transport studies, guided formation of cell clusters, and tissue engineering.
语言:: eng
DOI:: 10.1021/ac902596b

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