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PMID: 20163564 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Legionella pneumophila promotes functional interactions between plasma membrane syntaxins and Sec22b.

Traffic (Copenhagen, Denmark) ·Vol. 11 ·No. 5 ·2010-05-00 ·Pages 587-600

Arasaki K, Roy CR

Abstract

Biogenesis of a specialized organelle that supports intracellular replication of Legionella pneumophila involves the fusion of secretory vesicles exiting the endoplasmic reticulum (ER) with phagosomes containing this bacterial pathogen. Here, we investigated host plasma membrane SNARE proteins to determine whether they play a role in trafficking of vacuoles containing L. pneumophila. Depletion of plasma membrane syntaxins by RNA interference resulted in delayed acquisition of the resident ER protein calnexin and enhanced retention of Rab1 on phagosomes containing virulent L. pneumophila, suggesting that these SNARE proteins are involved in vacuole biogenesis. Plasma membrane-localized SNARE proteins syntaxin 2, syntaxin 3, syntaxin 4 and SNAP23 localized to vacuoles containing L. pneumophila. The ER-localized SNARE protein Sec22b was found to interact with plasma membrane SNAREs on vacuoles containing virulent L. pneumophila, but not on vacuoles containing avirulent mutants of L. pneumophila. The addition of alpha-SNAP and N-ethylmaleimide-sensitive factor (NSF) to the plasma membrane SNARE complexes formed by virulent L. pneumophila resulted in the dissociation of Sec22b, indicating functional pairing between these SNAREs. Thus, L. pneumophila stimulates the non-canonical pairing of plasma membrane t-SNAREs with the v-SNARE Sec22b to promote fusion of the phagosome with ER-derived vesicles. The mechanism by which L. pneumophila promotes pairing of plasma membrane syntaxins and Sec22b could provide unique insight into how the secretory vesicles could provide an additional membrane reserve subverted during phagosome maturation.

MeSH Terms
Calnexin/metabolism Cell Membrane/metabolism Endoplasmic Reticulum/metabolism Ethylmaleimide/metabolism Legionella pneumophila/metabolism Membrane Proteins/metabolism Microsomes/metabolism N-Ethylmaleimide-Sensitive Proteins/metabolism Organelles/metabolism Phagosomes/metabolism,microbiology Protein Transport Qa-SNARE Proteins/metabolism SNARE Proteins/metabolism Secretory Vesicles/metabolism Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins/metabolism Vacuoles/metabolism,microbiology
Chemicals
Membrane Proteins Qa-SNARE Proteins SNARE Proteins Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins Calnexin N-Ethylmaleimide-Sensitive Proteins Ethylmaleimide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Arasaki Kohei
Section of Microbial Pathogenesis, Yale University School of Medicine, Boyer Center for Molecular Medicine, New Haven, CT 06536, USA.
Roy Craig R
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Article Info
Journal
Traffic (Copenhagen, Denmark)
Abbr.
Traffic
ISSN
1600-0854
DOI
10.1111/j.1600-0854.2010.01050.x
Published
2010-05-00
Epub
2010-00-15
Pages
587-600
Language
English
Region
England
NLM ID
100939340
PMCID
PMC3164831
Subset
IM
Grants
NIAID NIH HHS · R01 AI041699 · United States
NIAID NIH HHS · R01 AI041699-15 · United States
NIAID NIH HHS · R29 AI041699 · United States
NIAID NIH HHS · R01AI041699 · United States
External Links
PubMed source DOI: 10.1111/j.1600-0854.2010.01050.x PMC Full Text View journal details
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