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PMID: 2014974 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Contribution of the cell-surface-associated enzyme in the Zimmermann-Rosselet assay of outer membrane permeability of beta-lactam antibiotics.

Antimicrobial agents and chemotherapy ·Vol. 35 ·No. 1 ·1991-01-00 ·Pages 177-9

Liu W, Nikaido H

Abstract

Analysis of beta-lactam hydrolysis by intact cells of several strains of enteric bacteria suggests that a significant fraction of beta-lactamase molecules is present at a location directly accessible for the substrates in the medium. We propose a method to correct for the error introduced by these "surface-exposed" enzymes in the Zimmermann-Rosselet assay of outer membrane permeability.

MeSH Terms
Anti-Bacterial Agents/pharmacokinetics Cell Membrane/enzymology Cell Membrane Permeability/physiology Gram-Negative Bacteria/enzymology,ultrastructure Kinetics beta-Lactamases/metabolism beta-Lactams
Chemicals
Anti-Bacterial Agents beta-Lactams beta-Lactamases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Liu W
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Nikaido H
References (7)
7 references, click to expand
  1. Outer membrane of Salmonella typhimurium: chemical analysis and freeze-fracture studies with lipopolysaccharide mutants.
    J Bacteriol. 1975 Nov;124(2):942-58 PMID: 1102538
  2. Function of the outer membrane of Escherichia coli as a permeability barrier to beta-lactam antibiotics.
    Antimicrob Agents Chemother. 1977 Sep;12(3):368-72 PMID: 334063
  3. Porin channels in Escherichia coli: studies with beta-lactams in intact cells.
    J Bacteriol. 1983 Jan;153(1):232-40 PMID: 6294048
  4. Sensitivity of Escherichia coli to various beta-lactams is determined by the interplay of outer membrane permeability and degradation by periplasmic beta-lactamases: a quantitative predictive treatment.
    Mol Microbiol. 1987 Jul;1(1):29-36 PMID: 3330755
  5. The permeability parameter of the outer membrane of Pseudomonas aeruginosa varies with the concentration of a test substrate, cephalosporin C.
    J Gen Microbiol. 1986 Jan;132(1):27-33 PMID: 3086492
  6. The time course of hydrolysis of a beta-lactam antibiotic by intact gram-negative bacteria possessing a periplasmic beta-lactamase.
    Biochem J. 1987 Jun 15;244(3):509-13 PMID: 3128262
  7. Role of permeability barriers in resistance to beta-lactam antibiotics.
    Pharmacol Ther. 1985;27(2):197-231 PMID: 2412244
Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.35.1.177
Published
1991-01-00
Pages
177-9
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC244962
Subset
IM
Grants
NIAID NIH HHS · AI-09644 · United States
External Links
PubMed source DOI: 10.1128/AAC.35.1.177 PMC Full Text View journal details
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