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PMID: 2014246 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Production of site-selected neutralizing human monoclonal antibodies against the third variable domain of the human immunodeficiency virus type 1 envelope glycoprotein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 8 ·1991-04-15 ·Pages 3238-42

Gorny MK, Xu JY, Gianakakos V, Karwowska S, Williams C, Sheppard HW, Hanson CV, Zolla-Pazner S

Abstract

Cell lines secreting IgG1 human monoclonal antibodies (mAb) to the envelope glycoprotein, gp120, of human immunodeficiency virus (HIV) have been produced by transformation of peripheral blood cells from HIV-infected individuals and by fusion of transformed cells to a human-mouse heteromyeloma cell line (SHM-D33). Two human mAbs were site-selected by means of a 23-mer synthetic peptide spanning a portion of the third variable domain of gp120 from the MN strain of HIV. The two heterohybridomas produce three times more IgG than do their parent lymphoblastoid cell lines. The specificities of these mAbs have been mapped to sequences near the tip of the disulfide loop of the gp120 third variable domain, Lys-Arg-Ile-His-Ile and His-Ile-Gly-Pro-Gly-Arg, respectively. The mAbs have dissociation constants of 3.7 x 10(-6) M and 8.3 x 10(-7) M, neutralize HIVMN in vitro at nanogram levels, and bear the characteristics of antibodies associated with protective immunity in vivo.

MeSH Terms
Antibodies, Monoclonal/immunology Antibody Affinity Antibody Specificity HIV Antibodies/immunology HIV Envelope Protein gp120/immunology HIV Seropositivity/immunology HIV-1/immunology Humans Neutralization Tests
Chemicals
Antibodies, Monoclonal HIV Antibodies HIV Envelope Protein gp120
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Gorny M K
Department of Pathology, New York University Medical Center, NY 10016.
Xu J Y
Gianakakos V
Karwowska S
Williams C
Sheppard H W
Hanson C V
Zolla-Pazner S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.8.3238
Published
1991-04-15
Pages
3238-42
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51421
Subset
IM
Grants
NIAID NIH HHS · AI 72658 · United States
NIAID NIH HHS · AI 82693 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.8.3238 PMC Full Text View journal details
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