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PMID: 2013576 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The virC and virD operons of the Agrobacterium Ti plasmid are regulated by the ros chromosomal gene: analysis of the cloned ros gene.

Journal of bacteriology ·Vol. 173 ·No. 8 ·1991-04-00 ·Pages 2608-16

Cooley MB, D'Souza MR, Kado CI

Abstract

The ros chromosomal gene is present in octopine and nopaline strains of Agrobacterium tumefaciens as well as in Rhizobium meliloti. This gene encodes a 15.5-kDa protein that specifically represses the virC and virD operons in the virulence region of the Ti plasmid. The ros gene was cloned from a genomic bank by electroporation and complementation in Agrobacterium cells. Reporter fusion to the ros gene indicates that the level of transcription is controlled in part by autoregulation. A consensus inverted repeat sequence present in the ros promoter and in the virC and virD promoters of pTiC58, pTiA6, and pRiA4b suggests that a specific Ros binding site exists in these promoters. In the virC and virD promoter region, this binding site is within a cluster of vir box consensus sequences in which the VirG protein binds. This suggests possible binding competition between Ros and VirG at the virC and virD promoters. That the Ros protein binds DNA is suggested by the presence of a 'zinc finger' consensus sequence in the protein.

Related Genes
ros virC virD
MeSH Terms
Amino Acid Sequence Bacterial Proteins/biosynthesis Base Sequence Blotting, Southern Chromosome Deletion Cloning, Molecular DNA/analysis Escherichia coli/genetics Gene Expression Regulation, Bacterial Molecular Sequence Data Operon/genetics Plasmids Promoter Regions, Genetic/genetics Proto-Oncogene Proteins/genetics,physiology Receptor Protein-Tyrosine Kinases Restriction Mapping Rhizobium/genetics Sequence Homology, Nucleic Acid Transcription, Genetic Virulence Factors Zinc Fingers/genetics
Chemicals
Bacterial Proteins Proto-Oncogene Proteins Virulence Factors DNA Receptor Protein-Tyrosine Kinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cooley M B
Davis Crown Gall Group, University of California, Davis 95616.
D'Souza M R
Kado C I
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.8.2608-2616.1991
Published
1991-04-00
Pages
2608-16
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207827
Subset
IM
Grants
NCI NIH HHS · CA-11526-21 · United States
NIGMS NIH HHS · GM45550 · United States
Databases
GENBANK
M55488, M55489, M55490, M55491, M55492, M55493, M55494, M65201, M93063, M93064
External Links
PubMed source DOI: 10.1128/jb.173.8.2608-2616.1991 PMC Full Text View journal details
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