Abstract
Splicing regulatory proteins often have distinct activities when bound to exons versus introns. However, less clear is whether variables aside from location can influence activity. HnRNP L binds to a motif present in both CD45 variable exons 4 and 5 to affect their coordinate repression. Here, we show that, in contrast to its direct repression of exon 4, hnRNP L represses exon 5 by countering the activity of a neighboring splicing enhancer. In the absence of the enhancer, hnRNP L unexpectedly activates exon inclusion. As the splice sites flanking exon 4 and 5 are distinct, we directly examined the effect of varying splice site strength on the mechanism of hnRNP L function. Remarkably, binding of hnRNP L to an exon represses strong splice sites but enhances weak splice sites. A model in which hnRNP L stabilizes snRNP binding can explain both effects in a manner determined by the inherent snRNP-substrate affinity.
MeSH Terms
Exons
Heterogeneous-Nuclear Ribonucleoprotein L/physiology
Humans
Leukocyte Common Antigens/chemistry,genetics,metabolism
Models, Genetic
RNA Splice Sites
RNA Splicing
Chemicals
Heterogeneous-Nuclear Ribonucleoprotein L
RNA Splice Sites
Leukocyte Common Antigens
PTPRC protein, human
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Motta-Mena Laura B
Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390-9038, USA.
Heyd Florian
Lynch Kristen W
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