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PMID: 20107022 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

The submergence tolerance regulator Sub1A mediates stress-responsive expression of AP2/ERF transcription factors.

Plant physiology ·Vol. 152 ·No. 3 ·2010-03-00 ·Pages 1674-92

Jung KH, Seo YS, Walia H, Cao P, Fukao T, Canlas PE, Amonpant F, Bailey-Serres J, Ronald PC

Abstract

We previously characterized the rice (Oryza sativa) Submergence1 (Sub1) locus encoding three ethylene-responsive factor (ERF) transcriptional regulators. Genotypes carrying the Sub1A-1 allele are tolerant of prolonged submergence. To elucidate the mechanism of Sub1A-1-mediated tolerance, we performed transcriptome analyses comparing the temporal submergence response of Sub1A-1-containing tolerant M202(Sub1) with the intolerant isoline M202 lacking this gene. We identified 898 genes displaying Sub1A-1-dependent regulation. Integration of the expression data with publicly available metabolic pathway data identified submergence tolerance-associated pathways governing anaerobic respiration, hormone responses, and antioxidant systems. Of particular interest were a set of APETALA2 (AP2)/ERF family transcriptional regulators that are associated with the Sub1A-1-mediated response upon submergence. Visualization of expression patterns of the AP2/ERF superfamily members in a phylogenetic context resolved 12 submergence-regulated AP2/ERFs into three putative functional groups: (1) anaerobic respiration and cytokinin-mediated delay in senescence via ethylene accumulation during submergence (three ERFs); (2) negative regulation of ethylene-dependent gene expression (five ERFs); and (3) negative regulation of gibberellin-mediated shoot elongation (four ERFs). These results confirm that the presence of Sub1A-1 impacts multiple pathways of response to submergence.

MeSH Terms
Acclimatization Cluster Analysis Floods Gene Expression Profiling Gene Expression Regulation, Plant Hydrogen Peroxide/analysis Multigene Family Oligonucleotide Array Sequence Analysis Oryza/genetics,physiology Phenotype Phylogeny Plant Proteins/genetics,metabolism Promoter Regions, Genetic Transcription Factors/genetics,metabolism Water
Chemicals
Plant Proteins Transcription Factors Water Hydrogen Peroxide
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Jung Ki-Hong
Department of Plant Pathology, University of California, Davis, California 95616, USA.
Seo Young-Su
Walia Harkamal
Cao Peijian
Fukao Takeshi
Canlas Patrick E
Amonpant Fawn
Bailey-Serres Julia
Ronald Pamela C
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
1532-2548
DOI
10.1104/pp.109.152157
Published
2010-03-00
Epub
2010-00-27
Pages
1674-92
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC2832257
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.109.152157 PMC Full Text View journal details
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