Purification and properties of an organophosphorus acid anhydrase from a halophilic bacterial isolate.

Journal of bacteriology ·Vol. 173 ·No. 6 ·1991-03-00 ·Pages 1938-43

DeFrank JJ, Cheng TC

Abstract

A moderately halophilic bacterial isolate has been found to possess high levels of enzymatic activity against several highly toxic organophosphorus compounds. The predominant enzyme, designated organophosphorus acid anhydrase 2, has been purified 1,000-fold to homogeneity and characterized. The enzyme is a single polypeptide with a molecular weight of 60,000. With diisopropylfluorophosphate as a substrate, the enzyme has optimum activity at pH 8.5 and 50 degrees C, and it is stimulated by manganese and cobalt.

MeSH Terms

Antibodies, Monoclonal/immunology Bacterial Proteins/immunology,isolation & purification Blotting, Western Chromatography Esterases Gram-Negative Bacteria/enzymology,immunology Hydrogen-Ion Concentration Hydrolases/antagonists & inhibitors,isolation & purification,metabolism Kinetics Metals/pharmacology Molecular Weight Organophosphorus Compounds/metabolism Phosphoric Triester Hydrolases Substrate Specificity Sulfhydryl Reagents/pharmacology Temperature

Chemicals

Antibodies, Monoclonal Bacterial Proteins Metals Organophosphorus Compounds Sulfhydryl Reagents diisopropylphosphate Hydrolases Esterases Phosphoric Triester Hydrolases diisopropyl-fluorophosphatase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

DeFrank J J

U.S. Army Chemical Research, Development & Engineering Center, Aberdeen Proving Ground, Maryland 21010-5423.

Cheng T C

References (4)

4 references, click to expand

Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Kinetics of the DFPase activity in Tetrahymena thermophila.
J Protozool. 1986 May;33(2):216-8 PMID: 3735152
Inhibition of a soman- and diisopropyl phosphorofluoridate (DFP)-detoxifying enzyme by Mipafox.
Biochem Pharmacol. 1985 Jun 15;34(12):2069-72 PMID: 4004925
Hydrolysis of nerve gas by squid-type diisopropyl phosphorofluoridate hydrolyzing enzyme on agarose resin.
Science. 1982 Mar 5;215(4537):1255-7 PMID: 7058344

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.173.6.1938-1943.1991

Published

1991-03-00

Pages

1938-43

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC207724

Subset

External Links

PubMed source DOI: 10.1128/jb.173.6.1938-1943.1991 PMC Full Text View journal details

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Purification and properties of an organophosphorus acid anhydrase from a halophilic bacterial isolate.

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