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PMID: 1999484 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Mucin production by human colonic carcinoma cells correlates with their metastatic potential in animal models of colon cancer metastasis.

The Journal of clinical investigation ·Vol. 87 ·No. 3 ·1991-03-00 ·Pages 1037-45

Bresalier RS, Niv Y, Byrd JC, Duh QY, Toribara NW, Rockwell RW, Dahiya R, Kim YS

Abstract

Patients with mucinous colorectal cancers characteristically present with advanced disease, however, the relationship between mucin production by colon cancer cells and their metastatic potential remains unclear. We therefore sought to define the relationship between mucin production by human colon cancer cells and metastatic ability by employing animal models of colon cancer metastasis. LS LiM 6, a colon carcinoma cell line with high liver metastasizing ability during cecal growth in nude mice produced twofold more metabolically labeled intracellular mucin and secreted four- to fivefold more mucin into the culture medium compared to poorly metastatic parental line LS174T. This was accompanied by a similar elevation in poly(A)+ RNA detected by blot hybridization with a human intestinal mucin cDNA probe, and increases in mucin core carbohydrate antigens determined immunohistochemically. Variants of LS174T selected for high (HM 7) or low (LM 12) mucin synthesizing capacity also yielded metastases after cecal growth and colonized the liver after splenic-portal injection in proportion to their ability to produce mucin. Inhibition of mucin glycosylation by the arylglycoside benzyl-alpha-N-acetyl-galactosamine greatly reduced liver colonization after splenic-portal injection of the tumor cells. These data suggest that mucin production by human colon cancer cells correlates with their metastatic potential and affects their ability to colonize the liver in experimental model systems.

MeSH Terms
Animals Blotting, Northern Carcinoma/metabolism,pathology Colonic Neoplasms/metabolism,pathology Glycosylation Humans Immunoenzyme Techniques Liver Neoplasms/pathology,secondary Mice Mice, Nude Mucins/biosynthesis,genetics Neoplasm Metastasis Neoplasm Transplantation RNA, Messenger/genetics Transplantation, Heterologous
Chemicals
Mucins RNA, Messenger
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Bresalier R S
GI Research Lab VA Medical Center, San Francisco, CA 94121.
Niv Y
Byrd J C
Duh Q Y
Toribara N W
Rockwell R W
Dahiya R
Kim Y S
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI115063
Published
1991-03-00
Pages
1037-45
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC329898
Subset
IM
Grants
NCI NIH HHS · CA45967 · United States
External Links
PubMed source DOI: 10.1172/JCI115063 PMC Full Text View journal details
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