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PMID: 1996337 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of lipid domains in erythrocyte membranes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 4 ·1991-02-15 ·Pages 1364-8

Rodgers W, Glaser M

Abstract

Fluorescence digital imaging microscopy was used to study the lateral distribution of the lipid components in erythrocyte membranes. Intact erythrocytes labeled with phospholipids containing a fluorophore attached to one fatty acid chain showed an uneven distribution of the phospholipids in the membrane thereby demonstrating the presence of membrane domains. The enrichment of the lipotropic compound chlor-promazine in domains in intact erythrocytes also suggested that the domains are lipid-enriched regions. Similar membrane domains were present in erythrocyte ghosts. The phospholipid enrichment was increased in the domains by inducing membrane protein aggregation. Double-labeling experiments were done to determine the relative distributions of different phospholipids in the membrane. Vesicles made from extracted lipids did not show the presence of domains consistent with the conclusion that membrane proteins were responsible for creating the domains. Overall, it was found that large domains exist in the red blood cell membrane with unequal enrichment of the different phospholipid species.

MeSH Terms
4-Chloro-7-nitrobenzofurazan Animals Erythrocyte Membrane/ultrastructure Membrane Lipids/blood Microscopy, Fluorescence Phospholipids/blood Rabbits
Chemicals
Membrane Lipids Phospholipids 4-Chloro-7-nitrobenzofurazan
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rodgers W
Department of Biochemistry, University of Illinois, Urbana 61801.
Glaser M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.4.1364
Published
1991-02-15
Pages
1364-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51018
Subset
IM
Grants
NIGMS NIH HHS · GM21953 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.4.1364 PMC Full Text View journal details
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