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PMID: 198748 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Methylated constituents of Aedes albopictus poly (A)-containing messenger RNA.

Nucleic acids research ·Vol. 4 ·No. 8 ·1977-08-00 ·Pages 2671-82

HsuChen CC, Dubin DT

Abstract

Poly (A)-containing mRNA prepared from cultured mosquito (Aedes albopictus) cells was found to contain methylated 5'-terminal "caps" as well as internal m6A residues. Both type I [m7G(5')ppp(5')Xmp] and type II [m7G(5')ppp(5')XmpYmp] caps were present, at molar ratio of ca five to one. All four common RNA bases were represented in the second position (Xm) of the caps, adenine being the most abundant and N6-methyladenine being absent. The four bases were also represented in the third position (Ym), but here uracil was the predominant base. There was approximately one internal m6A residue for every three caps. These studies demonstrate that mRNA from an invertebrate source can have a methylation pattern comparable with that of mammalian cells in it complexity.

MeSH Terms
Adenine/analogs & derivatives Aedes/analysis Alkaline Phosphatase Animals Cells, Cultured Floxuridine Methylation Phosphoric Diester Hydrolases Poly A/analysis RNA, Messenger Ribonucleases Ribonucleotides/analysis Ribose/analogs & derivatives
Chemicals
RNA, Messenger Ribonucleotides Floxuridine Poly A Ribose Ribonucleases Alkaline Phosphatase Phosphoric Diester Hydrolases Adenine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
HsuChen C C
Dubin D T
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23 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/4.8.2671
Published
1977-08-00
Pages
2671-82
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342600
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/4.8.2671 PMC Full Text View journal details
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