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PMID: 1987129 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Genetic transformation in Streptococcus pneumoniae: nucleotide sequence analysis shows comA, a gene required for competence induction, to be a member of the bacterial ATP-dependent transport protein family.

Journal of bacteriology ·Vol. 173 ·No. 1 ·1991-01-00 ·Pages 372-81

Hui FM, Morrison DA

Abstract

The complete nucleotide sequence of comA, a gene required for induction of competence for genetic transformation in Streptococcus pneumoniae, was determined by using plasmid DNA templates and synthetic oligonucleotide primers. The sequence contained a single large open reading frame, ORF1, of 2,151 bp. ORF1 was included within the comAB locus previously mapped genetically and accounted for 50% of its extent. The predicted molecular weight of the largest polypeptide encoded within ORF1, 80,290, coincided with that measured previously (77,000) for the product of in vitro transcription-translation of the cloned comA locus. A Shine-Dalgarno sequence (AAAGGAG, delta G = -14 kcal) lay immediately upstream of ORF1. A sequence (TTtAat-17 bp-TAaAAT) similar to the Escherichia coli sigma 70 promoter consensus was located 410 bp upstream of ORF1. The deduced protein sequence of ComA showed a very strong similarity to the E. coli hemolysin secretion protein, HlyB, and strong similarities to other members of the family of ATP-dependent transport proteins, including the mammalian multidrug resistance P-glycoprotein. These similarities suggest that ComA functions in the transport of some molecule, possibly pneumococcal competence factor itself.

Related Genes
comA
MeSH Terms
Adenosine Triphosphate/metabolism Amino Acid Sequence Bacterial Proteins/genetics,metabolism Base Sequence Biological Transport Carrier Proteins/genetics DNA-Binding Proteins Genes, Bacterial Molecular Sequence Data Open Reading Frames Restriction Mapping Sequence Homology, Nucleic Acid Streptococcus pneumoniae/genetics,metabolism Transformation, Bacterial
Chemicals
Bacterial Proteins Carrier Proteins ComA protein, Bacteria DNA-Binding Proteins Adenosine Triphosphate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hui F M
Department of Biological Sciences, University of Illinois, Chicago 60680.
Morrison D A
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.1.372-381.1991
Published
1991-01-00
Pages
372-81
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207196
Subset
IM
Databases
GENBANK
M31680, M36180, M62937, M62938, M62939, M62940, M62941, M62942, M62943, M62944, M62945
External Links
PubMed source DOI: 10.1128/jb.173.1.372-381.1991 PMC Full Text View journal details
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