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PMID: 19868749 Published · ppublish English Journal Article

STABLE SUSPENSIONS OF AUTOAGGLUTINABLE BACTERIA.

The Journal of experimental medicine ·Vol. 37 ·No. 5 ·1923-04-30 ·Pages 647-51

De Kruif PH, Northrop JH

Abstract

Autoagglutinating cultures of Type G, bacillus of rabbit septicemia, can be rendered stable by suspension in glycocoll-acetate-phosphate buffer mixtures pH 7.5 and 7.1. Distilled water suspensions can also be employed if the normal and immune serum under test is diluted with buffers of this pH instead of with 0.85 per cent NaCl. Five intensely autoagglutinable strains of Streptococcus haemolyticus have been stabilized by washing and by suspending in 0.001 N NaOH. Specific agglutination of these stable suspensions by rabbit antistreptococcus serum could be demonstrated by diluting the serum with M/320 NaCl.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
De Kruif P H
Laboratories of The Rockefeller Institute for Medical Research.
Northrop J H
References (2)
2 references, click to expand
  1. STUDIES ON THE BIOLOGY OF STREPTOCOCCUS : I. ANTIGENIC RELATIONSHIPS BETWEEN STRAINS OF STREPTOCOCCUS HAEMOLYTICUS.
    J Exp Med. 1919 Sep 1;30(3):179-213 PMID: 19868354
  2. RABBIT SEPTICEMIA BACILLUS, TYPES D AND G, IN NORMAL RABBITS.
    J Exp Med. 1922 Aug 31;36(3):309-16 PMID: 19868673
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
Published
1923-04-30
Pages
647-51
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2128352
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