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PMID: 1986216 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Isolation and characterization of PKC-L, a new member of the protein kinase C-related gene family specifically expressed in lung, skin, and heart.

Molecular and cellular biology ·Vol. 11 ·No. 1 ·1991-01-00 ·Pages 126-33

Bacher N, Zisman Y, Berent E, Livneh E

Abstract

We have isolated and characterized a new human cDNA, coding for a protein kinase, related to the protein kinase C (PKC) gene family. Although this protein kinase shares some homologous sequences and structural features with the four members of the PKC family initially isolated (alpha, beta I, beta II, and gamma), it shows more homology with the recently described PKC-related subfamily, encoded by the cDNAs delta, epsilon, and zeta. The transcript for this gene product, termed PKC-L, is most abundant in lung tissue, less expressed in heart and skin tissue, and exhibited very low expression in brain tissue. Thus, its tissue distribution is different from that described for other mammalian members of the PKC gene family, their expression being enriched in brain tissues. PKC-L is also expressed in several human cell lines, including the human epidermoid carcinoma line A431. The ability of phorbol esters to bind to and stimulate the kinase activity of PKC-L was revealed by introducing the cDNA into COS cells.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA/genetics Humans Lung/enzymology Molecular Sequence Data Myocardium/enzymology Phorbol Esters/metabolism Phosphorylation Precipitin Tests Protein Binding Protein Kinase C/classification,genetics,metabolism Recombinant Proteins/metabolism Restriction Mapping Skin/enzymology Transfection
Chemicals
Phorbol Esters Recombinant Proteins DNA Protein Kinase C
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bacher N
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
Zisman Y
Berent E
Livneh E
References (44)
44 references, click to expand
  1. Characterization of a specific phorbol ester aporeceptor in mouse brain cytosol.
    Proc Natl Acad Sci U S A. 1983 Jul;80(14):4208-12 PMID: 6308606
  2. Activation of calcium and phospholipid-dependent protein kinase by diacylglycerol, its possible relation to phosphatidylinositol turnover.
    J Biol Chem. 1980 Mar 25;255(6):2273-6 PMID: 7358670
  3. Preservation of morphological, functional, and karyotypic traits during long-term culture and in vivo passage of two human skin squamous cell carcinomas.
    Cancer Res. 1983 Dec;43(12 Pt 1):5995-6011 PMID: 6196111
  4. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.
    Nucleic Acids Res. 1984 Jan 25;12(2):857-72 PMID: 6694911
  5. Inositol trisphosphate, a novel second messenger in cellular signal transduction.
    Nature. 1984 Nov 22-28;312(5992):315-21 PMID: 6095092
  6. A novel mechanism of diglyceride formation. 12-O-tetradecanoylphorbol-13-acetate stimulates the cyclic breakdown and resynthesis of phosphatidylcholine.
    J Biol Chem. 1986 Jul 15;261(20):9128-32 PMID: 3087989
  7. Studies and perspectives of protein kinase C.
    Science. 1986 Jul 18;233(4761):305-12 PMID: 3014651
  8. Cloning and expression of multiple protein kinase C cDNAs.
    Cell. 1986 Aug 15;46(4):491-502 PMID: 3755379
  9. Large deletions in the cytoplasmic kinase domain of the epidermal growth factor receptor do not affect its laternal mobility.
    J Cell Biol. 1986 Aug;103(2):327-31 PMID: 3015981
  10. The complete primary structure of protein kinase C--the major phorbol ester receptor.
    Science. 1986 Aug 22;233(4766):853-9 PMID: 3755547
  11. Multiple, distinct forms of bovine and human protein kinase C suggest diversity in cellular signaling pathways.
    Science. 1986 Aug 22;233(4766):859-66 PMID: 3755548
  12. The heterogeneity of protein kinase C in various rat tissues.
    Biochem Biophys Res Commun. 1988 Mar 30;151(3):973-81 PMID: 3355565
  13. The structure, expression, and properties of additional members of the protein kinase C family.
    J Biol Chem. 1988 May 15;263(14):6927-32 PMID: 2834397
  14. A novel phorbol ester receptor/protein kinase, nPKC, distantly related to the protein kinase C family.
    Cell. 1988 Jun 3;53(5):731-41 PMID: 3370672
  15. Tissue distribution and developmental expression of protein kinase C isozymes.
    J Biol Chem. 1988 Jul 15;263(20):9868-73 PMID: 3133370
  16. The molecular heterogeneity of protein kinase C and its implications for cellular regulation.
    Nature. 1988 Aug 25;334(6184):661-5 PMID: 3045562
  17. The scanning model for translation: an update.
    J Cell Biol. 1989 Feb;108(2):229-41 PMID: 2645293
  18. Protein kinase C zeta subspecies from rat brain: its structure, expression, and properties.
    Proc Natl Acad Sci U S A. 1989 May;86(9):3099-103 PMID: 2470089
  19. Isolation and characterization of two new drosophila protein kinase C genes, including one specifically expressed in photoreceptor cells.
    Cell. 1989 May 5;57(3):403-12 PMID: 2720775
  20. Phorbol ester binding to protein kinase C requires a cysteine-rich zinc-finger-like sequence.
    Proc Natl Acad Sci U S A. 1989 Jul;86(13):4868-71 PMID: 2500657
  21. Activation of protein kinase C by naturally occurring ether-linked diglycerides.
    J Biol Chem. 1989 Aug 15;264(23):13818-24 PMID: 2760045
  22. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.
    Eur J Biochem. 1980 Jun;107(2):303-14 PMID: 6772444
  23. Current concepts and controversies in chemical carcinogenesis.
    J Supramol Struct Cell Biochem. 1981;17(2):99-120 PMID: 7033554
  24. Efficient infection of monkey cells with DNA of simian virus 40.
    Proc Natl Acad Sci U S A. 1981 Dec;78(12):7575-8 PMID: 6278479
  25. Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters.
    J Biol Chem. 1982 Jul 10;257(13):7847-51 PMID: 7085651
  26. Phorbol diester receptor copurifies with protein kinase C.
    Proc Natl Acad Sci U S A. 1983 Jan;80(1):36-40 PMID: 6296873
  27. Identification of a calcium- and phospholipid- dependent phorbol ester binding activity in the soluble fraction of mouse tissues.
    Biochem Biophys Res Commun. 1983 Feb 28;111(1):340-5 PMID: 6830598
  28. Two types of complementary DNAs of rat brain protein kinase C. Heterogeneity determined by alternative splicing.
    FEBS Lett. 1986 Oct 6;206(2):347-52 PMID: 2428667
  29. Tissue-specific expression of three distinct types of rabbit protein kinase C.
    Nature. 1987 Jan 8-14;325(7000):161-6 PMID: 3808073
  30. Isolation of cDNA clones encoding protein kinase C: evidence for a protein kinase C-related gene family.
    Proc Natl Acad Sci U S A. 1987 Feb;84(4):1065-9 PMID: 3469647
  31. Growth factors, oncogenes, and multistage carcinogenesis.
    J Cell Biochem. 1987 Mar;33(3):213-24 PMID: 3553218
  32. Expression and properties of two types of protein kinase C: alternative splicing from a single gene.
    Science. 1987 May 29;236(4805):1116-20 PMID: 3576226
  33. Structure and nucleotide sequence of a Drosophila melanogaster protein kinase C gene.
    EMBO J. 1987 Feb;6(2):433-41 PMID: 3107983
  34. Inositol trisphosphate and diacylglycerol: two interacting second messengers.
    Annu Rev Biochem. 1987;56:159-93 PMID: 3304132
  35. Alternative splicing increases the diversity of the human protein kinase C family.
    DNA. 1987 Oct;6(5):389-94 PMID: 3677994
  36. Protein kinase C contains a pseudosubstrate prototope in its regulatory domain.
    Science. 1987 Dec 18;238(4834):1726-8 PMID: 3686012
  37. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
    Science. 1988 Jan 29;239(4839):487-91 PMID: 2448875
  38. Overexpression of protein kinase C alpha-subtype in Swiss/3T3 fibroblasts causes loss of both high and low affinity receptor numbers for epidermal growth factor.
    J Biol Chem. 1990 Aug 5;265(22):13290-6 PMID: 1695906
  39. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  40. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  41. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  42. Calcium-dependent activation of a multifunctional protein kinase by membrane phospholipids.
    J Biol Chem. 1979 May 25;254(10):3692-5 PMID: 438153
  43. Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line.
    J Cell Biol. 1988 Mar;106(3):761-71 PMID: 2450098
  44. Zinc fingers: gilt by association.
    Cell. 1988 Jan 15;52(1):1-3 PMID: 3125980
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.1.126-133.1991
Published
1991-01-00
Pages
126-33
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359602
Subset
IM
Databases
GENBANK
M35697, M35698, M35699, M35700, M55284, M55285, M55286, M73693, M73694, M73695
Corrections
ErratumIn
PMID: 1545821
External Links
PubMed source DOI: 10.1128/mcb.11.1.126-133.1991 PMC Full Text View journal details
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