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PMID: 198581 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

[125I]deoxycytidine used in a rapid, sensitive, and specific assay for herpes simplex virus type 1 thymidine kinase.

Journal of virology ·Vol. 24 ·No. 1 ·1977-10-00 ·Pages 314-8

Summers WC, Summers WP

Abstract

[125I]deoxycytidine was a good substrate for herpes simplex virus type 1 thymidine kinase (TK), whereas [125I]deoxycytidine was a very poor substrate for cellular TK. Simple, sensitive, and specific assays for viral TK could be carried out in vivo and in vitro even in the presence of cell TK. Autoradiographic detection of incorporated [125I]deoxycytidine provided a rapid and simple method for detection of and screening for viral TK in infected as well as viral TK-transformed cells.

MeSH Terms
Cell Line DCMP Deaminase/antagonists & inhibitors Deoxycytidine Methods Simplexvirus/enzymology Substrate Specificity Tetrahydrouridine/pharmacology Thymidine Kinase/analysis,metabolism
Chemicals
Deoxycytidine Tetrahydrouridine Thymidine Kinase DCMP Deaminase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Summers W C
Summers W P
References (10)
10 references, click to expand
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  2. Transfer of thymidine kinase to thymidine kinaseless L cells by infection with ultraviolet-irradiated herpes simplex virus.
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  6. Deoxythymidine kinase induced in HeLa TK- cells by herpes simplex virus type I and type II. II. Purification and characterization.
    J Biol Chem. 1976 May 10;251(9):2605-10 PMID: 177418
  7. Possible peptide chain termination mutants in thymide kinase gene of a mammalian virus, herpes simplex virus.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):4081-4 PMID: 172894
  8. Gel electrophoresis and isoelectric focusing of mitochondrial and viral-induced thymidine kinases.
    Int J Cancer. 1974 Feb 15;13(2):203-18 PMID: 4817574
  9. Iodination of nucleic acids in vitro.
    Biochemistry. 1971 May 25;10(11):1993-2000 PMID: 4935105
  10. Studies of the enzymatic deamination of ara-cytidine. V. inhibition in vitro and in vivo by tetrahydrouridine and other reduced pyrimidine nucleosides.
    Biochem Pharmacol. 1968 Sep;17(9):1981-91 PMID: 4972143
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.24.1.314-318.1977
Published
1977-10-00
Pages
314-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515932
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.24.1.314-318.1977 PMC Full Text View journal details
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