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PMID: 19841137 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Cohesin SMC1beta protects telomeres in meiocytes.

The Journal of cell biology ·Vol. 187 ·No. 2 ·2009-10-19 ·Pages 185-99

Adelfalk C, Janschek J, Revenkova E, Blei C, Liebe B, Göb E, Alsheimer M, Benavente R, de Boer E, Novak I, Höög C, Scherthan H, Jessberger R

Abstract

Meiosis-specific mammalian cohesin SMC1beta is required for complete sister chromatid cohesion and proper axes/loop structure of axial elements (AEs) and synaptonemal complexes (SCs). During prophase I, telomeres attach to the nuclear envelope (NE), but in Smc1beta(-/-) meiocytes, one fifth of their telomeres fail to attach. This study reveals that SMC1beta serves a specific role at telomeres, which is independent of its role in determining AE/SC length and loop extension. SMC1beta is necessary to prevent telomere shortening, and SMC3, present in all known cohesin complexes, properly localizes to telomeres only if SMC1beta is present. Very prominently, telomeres in Smc1beta(-/-) spermatocytes and oocytes loose their structural integrity and suffer a range of abnormalities. These include disconnection from SCs and formation of large telomeric protein-DNA extensions, extended telomere bridges between SCs, ring-like chromosomes, intrachromosomal telomeric repeats, and a reduction of SUN1 foci in the NE. We suggest that a telomere structure protected from DNA rearrangements depends on SMC1beta.

MeSH Terms
Animals Cell Cycle Proteins/metabolism Chromosomal Proteins, Non-Histone/deficiency,metabolism Female Male Meiosis Mice Mice, Knockout Microscopy, Electron Oocytes/cytology,metabolism Spermatocytes/cytology,metabolism Telomere/metabolism
Chemicals
Cell Cycle Proteins Chromosomal Proteins, Non-Histone Smc1l2 protein, mouse cohesins
Authors & Affiliations
13 authors, click to expand affiliations / ORCID
Adelfalk Caroline
Institute of Physiological Chemistry, Medical Faculty Carl Gustav Carus, Dresden University of Technology, 01307 Dresden, Germany.
Janschek Johannes
Revenkova Ekaterina
Blei Cornelia
Liebe Bodo
Göb Eva
Alsheimer Manfred
Benavente Ricardo
de Boer Esther
Novak Ivana
Höög Christer
Scherthan Harry
Jessberger Rolf
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
1540-8140
DOI
10.1083/jcb.200808016
Published
2009-10-19
Pages
185-99
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2768837
Subset
IM
Grants
NIGMS NIH HHS · R01 GM062517 · United States
External Links
PubMed source DOI: 10.1083/jcb.200808016 PMC Full Text View journal details
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