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PMID: 19840933 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Aup1-mediated regulation of Rtg3 during mitophagy.

The Journal of biological chemistry ·Vol. 284 ·No. 51 ·2009-12-18 ·Pages 35885-95

Journo D, Mor A, Abeliovich H

Abstract

Mitophagy is an autophagic process that degrades mitochondria by an intracellular engulfment that leads to their delivery into the lumen of the cell's hydrolytic compartment, such as the lysosome in animal cells or the vacuole in yeast. It is hypothesized that such processes serve a quality control function to prevent or slow the accumulation of malfunctioning mitochondria, which are thought in turn to underlie central aspects of the aging process in eukaryotic organisms. We recently identified a conserved mitochondrial protein phosphatase homolog, Aup1, which is required for efficient stationary phase mitophagy in yeast. In the present report, we demonstrate that the retrograde signaling pathway (RTG) is defective in aup1Delta mutants. In agreement with a role for Aup1 in the regulation of the RTG pathway, we find that deletion of RTG3, a transcription factor that mediates the RTG response, causes a defect in stationary phase mitophagy and that deletion of AUP1 leads to changes in Rtg3 phosphorylation patterns under these conditions. In addition, we find that mitophagic conditions lead to induction of RTG pathway target genes in an Aup1-dependent fashion. Thus, our results suggest that the function of Aup1 in mitophagy could be explained through its regulation of Rtg3-dependent transcription.

MeSH Terms
Autophagy/physiology Basic Helix-Loop-Helix Leucine Zipper Transcription Factors/genetics,metabolism Gene Deletion Mitochondria/genetics,metabolism Phosphoprotein Phosphatases/genetics,metabolism Phosphorylation/physiology Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Signal Transduction/physiology Transcription, Genetic/physiology
Chemicals
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors RTG3 protein, S cerevisiae Saccharomyces cerevisiae Proteins PTC6 protein, S cerevisiae Phosphoprotein Phosphatases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Journo Dikla
Department of Biochemistry and Food Science, Faculty of Agricultural, Food, and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel.
Mor Angelika
Abeliovich Hagai
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Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
1083-351X
DOI
10.1074/jbc.M109.048140
Published
2009-12-18
Pages
35885-95
Language
English
Region
United States
NLM ID
2985121R
PMCID
PMC2791017
Subset
IM
External Links
PubMed source DOI: 10.1074/jbc.M109.048140 PMC Full Text View journal details
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