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题目:
Characterization of newly established bovine intestinal epithelial cell line.
作者:
Miyazawa(Kohtaro),Hondo(Tetsuya),Kanaya(Takashi),Tanaka(Sachi),Takakura(Ikuro),Itani(Wataru),Rose(Michael T),Kitazawa(Haruki),Yamaguchi(Takahiro),Aso(Hisashi)
状态:
发布时间2010-01-21 , 更新时间 2010-01-21
期刊:
Histochem Cell Biol
摘要:
Membranous epithelial cells (M cells) of the follicle-associated epithelium in Peyer's patches have a high capacity for transcytosis of several viruses and microorganisms. Here, we report that we have successfully established a bovine intestinal epithelial cell line (BIE cells) and developed an in vitro M cell model. BIE cells have a cobblestone morphology and microvilli-like structures, and strongly express cell-to-cell junctional proteins and cytokeratin, which is a specific intermediate filament protein of epithelial cells. After co-culture with murine intestinal lymphocytes or treatment with supernatant from bovine PBMC cultured with IL-2, BIE cells acquired the ability of transcytosis. Therefore, BIE cells have typical characteristics of bovine intestinal epithelial cells and also have the ability to differentiate into an M cell like linage. In addition, our results indicate that contact between immune cells and epithelial cells may not be absolutely required for the differentiation of M cells. We think that BIE cells will be useful for studying the transport mechanisms of various pathogens and also the evaluation of drug delivery via M cells.
语言:
eng
DOI:
10.1007/s00418-009-0648-3

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