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PMID: 19828744 Published · ppublish English Evaluation Study Journal Article Research Support, Non-U.S. Gov't

Enhanced viral etiological diagnosis of respiratory system infection outbreaks by use of a multitarget nucleic acid amplification assay.

Journal of clinical microbiology ·Vol. 47 ·No. 12 ·2009-12-00 ·Pages 3839-45

Wong S, Pabbaraju K, Lee BE, Fox JD

Abstract

A study was undertaken to assess the utility of the xTAG respiratory viral panel (RVP) for enhanced laboratory investigation of respiratory outbreaks. Specimens (n = 1,108) from 244 suspected respiratory virus outbreaks in 2006 and 2007 in Alberta, Canada, were included in the study. Testing by direct fluorescent antigen detection (DFA) and various in-house nucleic acid amplification tests (NATs) for common respiratory viruses provided an etiological diagnosis in 177 outbreaks (72.5%), with 524 samples testing positive (47.3%) for a respiratory virus. Two hundred samples from 51 unresolved outbreaks were further tested by RVP retrospectively. Fifty-eight samples from 30 unresolved outbreaks had a respiratory virus detected by RVP (47 picornavirus-positive, 9 coronavirus-positive, and 2 influenza virus A-positive samples). Overall, detection of a viral etiological agent was achieved in 90.8% of outbreaks using a combination of DFA, NATs, and RVP. Use of RVP enhances the laboratory investigation of respiratory virus outbreaks and facilitates appropriate patient and outbreak management.

MeSH Terms
Adolescent Adult Aged Aged, 80 and over Alberta Antigens, Viral/analysis Child Child, Preschool Disease Outbreaks Humans Infant Infant, Newborn Middle Aged Nucleic Acid Amplification Techniques/methods Respiratory System/virology Respiratory Tract Infections/diagnosis,virology Sensitivity and Specificity Virus Diseases/diagnosis,virology Viruses/classification,genetics,isolation & purification Young Adult
Chemicals
Antigens, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wong Sallene
Provincial Laboratory for Public Health (Microbiology), 3030 Hospital Drive NW, Calgary, Alberta T2N 4W4, Canada. S.Wong@provlab.ab.ca
Pabbaraju Kanti
Lee Bonita E
Fox Julie D
References (38)
38 references, click to expand
  1. Direct screening of clinical specimens for multiple respiratory pathogens using the Genaco Respiratory Panels 1 and 2.
    Diagn Mol Pathol. 2006 Sep;15(3):169-73 PMID: 16932073
  2. Human coronavirus OC43 causes influenza-like illness in residents and staff of aged-care facilities in Melbourne, Australia.
    Epidemiol Infect. 2005 Apr;133(2):273-7 PMID: 15816152
  3. Simultaneous detection and high-throughput identification of a panel of RNA viruses causing respiratory tract infections.
    J Clin Microbiol. 2007 Jul;45(7):2105-9 PMID: 17507510
  4. Respiratory virus surveillance and outbreak investigation.
    J Clin Virol. 2007 Nov;40 Suppl 1:S24-30 PMID: 18162251
  5. Adamantane resistance in circulating human influenza A viruses from Alberta, Canada (1970-2007).
    Antiviral Res. 2008 Aug;79(2):81-6 PMID: 18258311
  6. Treatment of respiratory virus infections.
    Antiviral Res. 2006 Jun;70(2):1-16 PMID: 16513187
  7. A novel neuraminidase deletion mutation conferring resistance to oseltamivir in clinical influenza A/H3N2 virus.
    J Infect Dis. 2009 Jan 15;199(2):180-3 PMID: 19046066
  8. Frequent detection of human rhinoviruses, paramyxoviruses, coronaviruses, and bocavirus during acute respiratory tract infections.
    J Med Virol. 2006 Sep;78(9):1232-40 PMID: 16847968
  9. Outbreak of human metapneumovirus infection in norwegian children.
    Pediatr Infect Dis J. 2004 May;23(5):436-40 PMID: 15131467
  10. An outbreak of severe respiratory tract infection due to human metapneumovirus in a long-term care facility.
    Clin Infect Dis. 2007 May 1;44(9):1152-8 PMID: 17407031
  11. Vaccine-preventable adenoviral respiratory illness in US military recruits, 1999-2004.
    Vaccine. 2006 Apr 5;24(15):2835-42 PMID: 16480793
  12. A diverse group of previously unrecognized human rhinoviruses are common causes of respiratory illnesses in infants.
    PLoS One. 2007 Oct 03;2(10):e966 PMID: 17912345
  13. Evaluation of multiple test methods for the detection of the novel 2009 influenza A (H1N1) during the New York City outbreak.
    J Clin Virol. 2009 Jul;45(3):191-5 PMID: 19540158
  14. Community-acquired viral pneumonia.
    Clin Geriatr Med. 2007 Aug;23(3):535-52, vi PMID: 17631232
  15. Detection of a broad range of human adenoviruses in respiratory tract samples using a sensitive multiplex real-time PCR assay.
    J Med Virol. 2008 May;80(5):856-65 PMID: 18360899
  16. Nucleic acid amplification tests for the detection and analysis of respiratory viruses: the future for diagnostics?
    Future Microbiol. 2007 Apr;2(2):199-211 PMID: 17661656
  17. Viral pneumonia in older adults.
    Clin Infect Dis. 2006 Feb 15;42(4):518-24 PMID: 16421796
  18. Development and evaluation of NucliSens basic kit NASBA for diagnosis of parainfluenza virus infection with 'end-point' and 'real-time' detection.
    J Virol Methods. 2003 Mar;108(2):145-55 PMID: 12609681
  19. Medical and economic impact of a respiratory syncytial virus outbreak in a neonatal intensive care unit.
    Pediatr Infect Dis J. 2005 Dec;24(12):1040-4 PMID: 16371862
  20. Long-term care facilities: a cornucopia of viral pathogens.
    J Am Geriatr Soc. 2008 Jul;56(7):1281-5 PMID: 18557966
  21. Enhanced identification of viral and atypical bacterial pathogens in lower respiratory tract samples with nucleic acid amplification tests.
    J Med Virol. 2006 May;78(5):702-10 PMID: 16555283
  22. Comparison of the Luminex xTAG respiratory viral panel with in-house nucleic acid amplification tests for diagnosis of respiratory virus infections.
    J Clin Microbiol. 2008 Sep;46(9):3056-62 PMID: 18632902
  23. Cost analysis of multiplex PCR testing for diagnosing respiratory virus infections.
    J Clin Microbiol. 2009 Sep;47(9):2812-7 PMID: 19571025
  24. Outbreak of human metapneumovirus infection in elderly inpatients in Japan.
    J Am Geriatr Soc. 2006 Jan;54(1):177-80 PMID: 16420227
  25. A summer outbreak of human metapneumovirus infection in a long-term-care facility.
    J Infect Dis. 2007 Sep 1;196(5):705-8 PMID: 17674312
  26. Development and evaluation of a real-time nucleic acid sequence based amplification assay for rapid detection of influenza A.
    J Med Virol. 2004 Dec;74(4):619-28 PMID: 15484279
  27. Nucleic acid amplification tests for detection of respiratory viruses.
    J Clin Virol. 2007 Nov;40 Suppl 1:S15-23 PMID: 18162249
  28. Evaluation of a multiplexed PCR assay for detection of respiratory viral pathogens in a public health laboratory setting.
    J Clin Microbiol. 2007 Dec;45(12):3875-82 PMID: 17928425
  29. Development of a respiratory virus panel test for detection of twenty human respiratory viruses by use of multiplex PCR and a fluid microbead-based assay.
    J Clin Microbiol. 2007 Sep;45(9):2965-70 PMID: 17596360
  30. Multi-year study of human metapneumovirus infection at a large US Midwestern Medical Referral Center.
    J Clin Virol. 2006 Dec;37(4):269-76 PMID: 17008122
  31. An outbreak of human metapneumovirus infection in hospitalized psychiatric adult patients in Taiwan.
    Scand J Infect Dis. 2009;41(5):363-7 PMID: 19308801
  32. The financial impact of controlling a respiratory virus outbreak in a teaching hospital: lessons learned from SARS.
    Can J Public Health. 2005 Jan-Feb;96(1):52-4 PMID: 15682697
  33. A novel group of rhinoviruses is associated with asthma hospitalizations.
    J Allergy Clin Immunol. 2009 Jan;123(1):98-104.e1 PMID: 19027147
  34. Update: influenza activity--United States, September 28, 2008-April 4, 2009, and composition of the 2009-10 influenza vaccine.
    MMWR Morb Mortal Wkly Rep. 2009 Apr 17;58(14):369-74 PMID: 19373198
  35. High-throughput, sensitive, and accurate multiplex PCR-microsphere flow cytometry system for large-scale comprehensive detection of respiratory viruses.
    J Clin Microbiol. 2007 Aug;45(8):2626-34 PMID: 17537928
  36. Detection of respiratory viruses using non-molecular based methods.
    J Clin Virol. 2007 Nov;40 Suppl 1:S11-4 PMID: 18162248
  37. Hospitalization attributable to influenza and other viral respiratory illnesses in Canadian children.
    Pediatr Infect Dis J. 2006 Sep;25(9):795-800 PMID: 16940836
  38. Diagnosis and epidemiological studies of human metapneumovirus using real-time PCR.
    J Clin Virol. 2007 Nov;40(3):186-92 PMID: 17870660
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
1098-660X
DOI
10.1128/JCM.01469-09
Email
s.wong@provlab.ab.ca
Published
2009-12-00
Epub
2009-00-14
Pages
3839-45
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC2786615
Subset
IM
External Links
PubMed source DOI: 10.1128/JCM.01469-09 PMC Full Text View journal details
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