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PMID: 19797662 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter.

Science (New York, N.Y.) ·Vol. 326 ·No. 5949 ·2009-10-02 ·Pages 144-7

Jiang D, Zhao L, Clapham DE

Abstract

Mitochondria are integral components of cellular calcium (Ca2+) signaling. Calcium stimulates mitochondrial adenosine 5'-triphosphate production, but can also initiate apoptosis. In turn, cytoplasmic Ca2+ concentrations are regulated by mitochondria. Although several transporter and ion-channel mechanisms have been measured in mitochondria, the molecules that govern Ca2+ movement across the inner mitochondrial membrane are unknown. We searched for genes that regulate mitochondrial Ca2+ and H+ concentrations using a genome-wide Drosophila RNA interference (RNAi) screen. The mammalian homolog of one Drosophila gene identified in the screen, Letm1, was found to specifically mediate coupled Ca2+/H+ exchange. RNAi knockdown, overexpression, and liposome reconstitution of the purified Letm1 protein demonstrate that Letm1 is a mitochondrial Ca2+/H+ antiporter.

MeSH Terms
Animals Antiporters/genetics,metabolism Calcium/metabolism Calcium-Binding Proteins/genetics,metabolism Cation Transport Proteins/genetics,metabolism Cell Line Drosophila Proteins/genetics,metabolism Drosophila melanogaster/genetics,metabolism Genome, Human Genome, Insect HeLa Cells Humans Hydrogen/metabolism Hydrogen-Ion Concentration Ion Transport Membrane Potential, Mitochondrial Membrane Proteins/genetics,metabolism Mitochondria/metabolism Mitochondrial Membranes/metabolism Mitochondrial Proteins/genetics,metabolism Proteolipids/metabolism RNA Interference
Chemicals
Antiporters Calcium-Binding Proteins Cation Transport Proteins Drosophila Proteins LETM1 protein, human Letm1 protein, Drosophila Membrane Proteins Mitochondrial Proteins Proteolipids calcium-hydrogen antiporters proteoliposomes Hydrogen Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jiang Dawei
Department of Cardiology, Howard Hughes Medical Institute, Children's Hospital Boston, Manton Center for Orphan Disease, and Department of Neurobiology, Harvard Medical School, Enders Building 1309, 320 Longwood Avenue, Boston, MA 02115, USA.
Zhao Linlin
Clapham David E
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Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
1095-9203
Published
2009-10-02
Pages
144-7
Language
English
Region
United States
NLM ID
0404511
PMCID
PMC4067766
Subset
IM
Grants
Howard Hughes Medical Institute · United States
Corrections
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