Abstract
Mitochondria are integral components of cellular calcium (Ca2+) signaling. Calcium stimulates mitochondrial adenosine 5'-triphosphate production, but can also initiate apoptosis. In turn, cytoplasmic Ca2+ concentrations are regulated by mitochondria. Although several transporter and ion-channel mechanisms have been measured in mitochondria, the molecules that govern Ca2+ movement across the inner mitochondrial membrane are unknown. We searched for genes that regulate mitochondrial Ca2+ and H+ concentrations using a genome-wide Drosophila RNA interference (RNAi) screen. The mammalian homolog of one Drosophila gene identified in the screen, Letm1, was found to specifically mediate coupled Ca2+/H+ exchange. RNAi knockdown, overexpression, and liposome reconstitution of the purified Letm1 protein demonstrate that Letm1 is a mitochondrial Ca2+/H+ antiporter.
MeSH Terms
Animals
Antiporters/genetics,metabolism
Calcium/metabolism
Calcium-Binding Proteins/genetics,metabolism
Cation Transport Proteins/genetics,metabolism
Cell Line
Drosophila Proteins/genetics,metabolism
Drosophila melanogaster/genetics,metabolism
Genome, Human
Genome, Insect
HeLa Cells
Humans
Hydrogen/metabolism
Hydrogen-Ion Concentration
Ion Transport
Membrane Potential, Mitochondrial
Membrane Proteins/genetics,metabolism
Mitochondria/metabolism
Mitochondrial Membranes/metabolism
Mitochondrial Proteins/genetics,metabolism
Proteolipids/metabolism
RNA Interference
Chemicals
Antiporters
Calcium-Binding Proteins
Cation Transport Proteins
Drosophila Proteins
LETM1 protein, human
Letm1 protein, Drosophila
Membrane Proteins
Mitochondrial Proteins
Proteolipids
calcium-hydrogen antiporters
proteoliposomes
Hydrogen
Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jiang Dawei
Department of Cardiology, Howard Hughes Medical Institute, Children's Hospital Boston, Manton Center for Orphan Disease, and Department of Neurobiology, Harvard Medical School, Enders Building 1309, 320 Longwood Avenue, Boston, MA 02115, USA.
Zhao Linlin
Clapham David E
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