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PMID: 19779621 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

Investigating the targets of MIR-15a and MIR-16-1 in patients with chronic lymphocytic leukemia (CLL).

PloS one ·Vol. 4 ·No. 9 ·2009-09-25 ·Pages e7169

Hanlon K, Rudin CE, Harries LW

Abstract

MicroRNAs (miRNAs) are short, noncoding RNAs that regulate the expression of multiple target genes. Deregulation of miRNAs is common in human tumorigenesis. The miRNAs, MIR-15a/16-1, at chromosome band 13q14 are down-regulated in the majority of patients with chronic lymphocytic leukaemia (CLL). We have measured the expression of MIR-15a/16-1, and 92 computationally-predicted MIR-15a/16-1 target genes in CLL patients and in normal controls. We identified 35 genes that are deregulated in CLL patients, 5 of which appear to be specific targets of the MIR-15a/16-1 cluster. These targets included 2 genes (BAZ2A and RNF41) that were significantly up-regulated (p<0.05) and 3 genes (RASSF5, MKK3 and LRIG1) that were significantly down-regulated (p<0.05) in CLL patients with down-regulated MIR-15a/16-1 expression. The genes identified here as being subject to MIR-15a/16-1 regulation could represent direct or indirect targets of these miRNAs. Many of these are good biological candidates for involvement in tumorigenesis and as such, may be important in the aetiology of CLL.

MeSH Terms
Chromosomes/ultrastructure Chromosomes, Human, Pair 13 Computational Biology/methods Down-Regulation Gene Expression Profiling Gene Expression Regulation, Leukemic Humans Immunophenotyping In Situ Hybridization, Fluorescence Leukemia, Lymphocytic, Chronic, B-Cell/genetics,metabolism MicroRNAs/biosynthesis RNA/metabolism
Chemicals
MIRN15 microRNA, human MIRN16 microRNA, human MicroRNAs RNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hanlon Katy
Department of Haematology, Royal Devon and Exeter NHS Foundation Trust, Exeter, Devon, United Kingdom.
Rudin Claudius E
Harries Lorna W
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0007169
Published
2009-09-25
Epub
2009-00-25
Pages
e7169
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC2745703
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pone.0007169 PMC Full Text View journal details
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