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PMID: 19772605 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

Transplantation of human neural stem cells transduced with Olig2 transcription factor improves locomotor recovery and enhances myelination in the white matter of rat spinal cord following contusive injury.

BMC neuroscience ·Vol. 10 ·2009-09-22 ·Pages 117

Hwang DH, Kim BG, Kim EJ, Lee SI, Joo IS, Suh-Kim H, Sohn S, Kim SU

Abstract

Contusive spinal cord injury is complicated by a delayed loss of oligodendrocytes, resulting in chronic progressive demyelination. Therefore, transplantation strategies to provide oligodendrocyte lineage cells and to enhance the extent of myelination appear to be justified for spinal cord repair. The present study investigated whether transplantation of human neural stem cells (NSCs) genetically modified to express Olig2 transcription factor, an essential regulator of oligodendrocyte development, can improve locomotor recovery and enhance myelination in a rat contusive spinal cord injury model. HB1.F3 (F3) immortalized human NSC line was transduced with a retroviral vector encoding Olig2, an essential regulator of oligodendrocyte development. Overexpression of Olig2 in human NSCs (F3.Olig2) induced activation of NKX2.2 and directed differentiation of NSCs into oligodendrocyte lineage cells in vitro. Introduction of Olig2 conferred higher proliferative activity, and a much larger number of F3.Olig2 NSCs were detected by 7 weeks after transplantation into contused spinal cord than that of parental F3 NSCs. F3.Olig2 NSCs exhibited frequent migration towards the white matter, whereas F3 NSCs were mostly confined to the gray matter or around the lesion cavities. Most of F3.Olig2 NSCs occupying the spared white matter differentiated into mature oligodendrocytes. Transplantation of F3.Olig2 NSCs increased the volume of spared white matter and reduced the cavity volume. Moreover, F3.Olig2 grafts significantly increased the thickness of myelin sheath around the axons in the spared white matter. Finally, animals with F3.Olig2 grafts showed an improvement in the quality of hindlimbs locomotion. Transplantation of NSCs genetically modified to differentiate into an oligodendrocytic lineage may be an effective strategy to improve functional outcomes following spinal cord trauma. The present study suggests that molecular factors governing cell fate decisions can be manipulated to enhance reparative potential of the cell-based therapy.

MeSH Terms
Analysis of Variance Animals Basic Helix-Loop-Helix Transcription Factors/genetics Cell Count Cell Differentiation Cell Proliferation Cells, Cultured Female Fetal Stem Cells/transplantation Genetic Vectors/genetics Homeobox Protein Nkx-2.2 Homeodomain Proteins Humans Image Processing, Computer-Assisted Immunohistochemistry Microscopy, Phase-Contrast Motor Activity Myelin Sheath/pathology Nerve Fibers, Myelinated/pathology Nerve Tissue Proteins/genetics Neurons/cytology,transplantation Nuclear Proteins Oligodendrocyte Transcription Factor 2 Rats Rats, Sprague-Dawley Recovery of Function Reverse Transcriptase Polymerase Chain Reaction Spinal Cord/pathology Spinal Cord Injuries/pathology,physiopathology,therapy Telencephalon/cytology Thoracic Vertebrae Transcription Factors Transfection
Chemicals
Basic Helix-Loop-Helix Transcription Factors Homeobox Protein Nkx-2.2 Homeodomain Proteins NKX2-2 protein, human Nerve Tissue Proteins Nkx2-2 protein, rat Nuclear Proteins OLIG2 protein, human Oligodendrocyte Transcription Factor 2 Transcription Factors
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Hwang Dong H
Brain Disease Research Center, Institute for Medical Sciences, Ajou University School of Medicine, Suwon, Korea. drhdh@ajou.ac.kr
Kim Byung G
Kim Eun J
Lee Seung I
Joo In S
Suh-Kim Haeyoung
Sohn Seonghyang
Kim Seung U
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Article Info
Journal
BMC neuroscience
Abbr.
BMC Neurosci
ISSN
1471-2202
DOI
10.1186/1471-2202-10-117
Email
drhdh@ajou.ac.kr
Published
2009-09-22
Epub
2009-00-22
Pages
117
Language
English
Region
England
NLM ID
100966986
PMCID
PMC2758886
Subset
IM
External Links
PubMed source DOI: 10.1186/1471-2202-10-117 PMC Full Text View journal details
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