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PMID: 1974216 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

HLA-DQ beta-chain restriction fragment length polymorphism as a risk marker in type 1 (insulin-dependent) diabetes mellitus: a Finnish family study.

Diabetologia ·Vol. 33 ·No. 6 ·1990-06-00 ·Pages 357-62

Reijonen H, Ilonen J, Knip M, Michelsen B, Akerblom HK

Abstract

Finnish Type 1 (insulin-dependent) diabetic families were analysed for HLA-DQ beta-chain polymorphism using a short intron-specific probe. A simple hybridization pattern was obtained in which all fragments were associated significantly with Type 1 diabetes. The simultaneous presence of two different risk markers, the allelic 12-kilobase and 4-kilobase fragments were strongly associated with Type 1 diabetes since 50% of the patients had this combination compared with only 2% of the control subjects. The cosegregated 7.5/3.0 kilobase fragments, which were associated with HLA-DR2 and DRw6 were not detected among the diabetic patients but were present in 48% of the control subjects. Our results provide further support for the location of susceptibility determining factors in the HLA-DQ gene area. The clear-cut, simple restriction fragment length polymorphism pattern obtained here, which bears a resemblance to a two allelic system, therefore makes this method applicable for estimating the risk of Type 1 diabetes at the population level.

MeSH Terms
Adolescent Alleles B-Lymphocytes/immunology Biomarkers/analysis Child Deoxyribonuclease BamHI Diabetes Mellitus, Type 1/genetics,immunology Finland Genotype HLA-DQ Antigens/genetics HLA-DQ beta-Chains HLA-DR Antigens/genetics Haplotypes Humans Polymorphism, Restriction Fragment Length Reference Values Risk Factors
Chemicals
Biomarkers HLA-DQ Antigens HLA-DQ beta-Chains HLA-DQbeta antigen HLA-DR Antigens Deoxyribonuclease BamHI
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Reijonen H
Department of Medical Microbiology, University of Oulu, Denmark.
Ilonen J
Knip M
Michelsen B
Akerblom H K
References (28)
28 references, click to expand
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Article Info
Journal
Diabetologia
Abbr.
Diabetologia
ISSN
0012-186X
DOI
10.1007/BF00404640
Published
1990-06-00
Pages
357-62
Language
English
Region
Germany
NLM ID
0006777
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00404640 View journal details
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