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PMID: 19740989 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Role of RNA structures in genome terminal sequences of the hepatitis C virus for replication and assembly.

Journal of virology ·Vol. 83 ·No. 22 ·2009-11-00 ·Pages 11989-95

Friebe P, Bartenschlager R

Abstract

Hepatitis C virus (HCV) is a positive-strand RNA virus replicating its genome via a negative-strand [(-)] intermediate. Little is known about replication signals residing in the 3' end of HCV (-) RNA. Recent studies identified seven stem-loop structures (SL-I', -IIz', -IIy', -IIIa', -IIIb', -IIIcdef', and -IV') in this region. In the present study, we mapped the minimal region required for RNA replication to SL-I' and -IIz', functionally confirmed the SL-IIz' structure, and identified SL-IIIa' to -IV' as auxiliary replication elements. In addition, we show that the 5' nontranslated region of the genome most likely does not contain cis-acting RNA structures required for RNA packaging into infectious virions.

MeSH Terms
Cell Line Hepacivirus/genetics,physiology Humans Mutagenesis, Site-Directed Nucleic Acid Conformation RNA, Viral/genetics,physiology Virus Assembly/genetics,physiology Virus Replication/genetics,physiology
Chemicals
RNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Friebe Peter
Department of Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany.
Bartenschlager Ralf
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
1098-5514
DOI
10.1128/JVI.01508-09
Published
2009-11-00
Epub
2009-00-09
Pages
11989-95
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC2772684
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.01508-09 PMC Full Text View journal details
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