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PMID: 1974051 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Structural and functional comparisons of the Drosophila virilis and Drosophila melanogaster rough genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 15 ·1990-08-00 ·Pages 5916-20

Heberlein U, Rubin GM

Abstract

We have isolated the homeobox gene rough (ro) from Drosophila virilis. Comparison of the predicted amino acid sequences of the D. melanogaster and D. virilis rough proteins reveals that domains of high conservation, including the homeodomain, are interspersed with highly diverged regions. Stretches of significant sequence conservation are also observed in the 5' promoter region and in the introns. The D. virilis rough gene rescues the rough mutant phenotype and is properly regulated when introduced into the D. melanogaster genome. Thus the rough protein as well as the cis-regulatory elements that ensure proper temporal and spatial regulation are functionally conserved between these Drosophila species.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Biological Evolution Cell Cycle Proteins DNA-Binding Proteins/genetics Drosophila/genetics Drosophila Proteins Drosophila melanogaster/genetics Genes, Homeobox Genetic Variation Genomic Library Introns Microtubule-Associated Proteins Molecular Sequence Data Promoter Regions, Genetic Sequence Homology, Nucleic Acid Transcription Factors/genetics
Chemicals
Cell Cycle Proteins DNA-Binding Proteins Drosophila Proteins Microtubule-Associated Proteins ROD protein, Drosophila Transcription Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Heberlein U
Howard Hughes Medical Institute, University of California, Berkeley 94720.
Rubin G M
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.15.5916
Published
1990-08-00
Pages
5916-20
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54440
Subset
IM
Databases
GENBANK
M35372
External Links
PubMed source DOI: 10.1073/pnas.87.15.5916 PMC Full Text View journal details
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