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PMID: 19738003 Published · ppublish English Journal Article Review

Lipoproteomics: using mass spectrometry-based proteomics to explore the assembly, structure, and function of lipoproteins.

Journal of lipid research ·Vol. 50 ·No. 10 ·2009-10-00 ·Pages 1967-75

Hoofnagle AN, Heinecke JW

Abstract

Lipoproteins are centrally important in lipid transport, fuel metabolism, and cardiovascular disease. The prototypic lipoprotein has an outer shell of amphipathic lipids and proteins that solubilizes a hydrophobic lipid core. Lipoprotein-associated proteins have classically been viewed as structural elements and factors important in lipid metabolism. Recent mass spectrometric analyses reveal that the protein cargo of lipoproteins is much more diverse than previously appreciated, raising the possibility that lipoproteins play previously unsuspected roles in host defense mechanisms and inflammation. They further suggest that lipoprotein-associated proteins can identify humans at increased risk of cardiovascular disease. Here, we summarize recent developments in lipoproteomics, the proteomic analysis of lipoproteins. We also discuss the promises and challenges this powerful analytical strategy offers for expanding our understanding of the biology and structures of lipoproteins.

MeSH Terms
Animals Cardiovascular Diseases/metabolism Humans Lipoproteins/chemistry,metabolism Mass Spectrometry/methods Models, Biological Proteomics/methods
Chemicals
Lipoproteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hoofnagle Andrew N
Department of Laboratory Medicine, University of Washington, Seattle, WA, USA. ahoof@u.washington.edu
Heinecke Jay W
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Article Info
Journal
Journal of lipid research
Abbr.
J Lipid Res
ISSN
1539-7262
DOI
10.1194/jlr.R900015-JLR200
Email
ahoof@u.washington.edu
Published
2009-10-00
Pages
1967-75
Language
English
Region
United States
NLM ID
0376606
PMCID
PMC2739765
Subset
IM
External Links
PubMed source DOI: 10.1194/jlr.R900015-JLR200 PMC Full Text View journal details
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