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PMID: 19698979 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Genome-wide mapping of HATs and HDACs reveals distinct functions in active and inactive genes.

Cell ·Vol. 138 ·No. 5 ·2009-09-04 ·Pages 1019-31

Wang Z, Zang C, Cui K, Schones DE, Barski A, Peng W, Zhao K

Abstract

Histone acetyltransferases (HATs) and deacetylases (HDACs) function antagonistically to control histone acetylation. As acetylation is a histone mark for active transcription, HATs have been associated with active and HDACs with inactive genes. We describe here genome-wide mapping of HATs and HDACs binding on chromatin and find that both are found at active genes with acetylated histones. Our data provide evidence that HATs and HDACs are both targeted to transcribed regions of active genes by phosphorylated RNA Pol II. Furthermore, the majority of HDACs in the human genome function to reset chromatin by removing acetylation at active genes. Inactive genes that are primed by MLL-mediated histone H3K4 methylation are subject to a dynamic cycle of acetylation and deacetylation by transient HAT/HDAC binding, preventing Pol II from binding to these genes but poising them for future activation. Silent genes without any H3K4 methylation signal show no evidence of being bound by HDACs.

MeSH Terms
Acetylation Cell Line Gene Expression Genome, Human Histone Acetyltransferases/genetics,metabolism Histone Deacetylase Inhibitors Histone Deacetylases/genetics,metabolism Histones/metabolism Humans Methylation Phosphorylation RNA Polymerase II/metabolism
Chemicals
Histone Deacetylase Inhibitors Histones Histone Acetyltransferases RNA Polymerase II Histone Deacetylases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Wang Zhibin
Laboratory of Molecular Immunology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Zang Chongzhi
Cui Kairong
Schones Dustin E
Barski Artem
Peng Weiqun
Zhao Keji
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
1097-4172
DOI
10.1016/j.cell.2009.06.049
Published
2009-09-04
Epub
2009-00-20
Pages
1019-31
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC2750862
Subset
IM
Grants
Intramural NIH HHS · Z01 HL005801-05 · United States
Databases
GEO
GSE15735
External Links
PubMed source DOI: 10.1016/j.cell.2009.06.049 PMC Full Text View journal details
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