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PMID: 19688112 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Chlamydia trachomatis alters iron-regulatory protein-1 binding capacity and modulates cellular iron homeostasis in HeLa-229 cells.

Journal of biomedicine & biotechnology ·Vol. 2009 ·2009-00-00 ·Pages 342032

Vardhan H, Bhengraj AR, Jha R, Singh Mittal A

Abstract

Chlamydia trachomatis (CT) is the leading cause of diseases related to reproductive health and iron plays important role in chlamydial pathogenesis. Iron homeostasis in chlamydia-infected cells is not clear thus far. This study shows that expression of the transferrin receptor (TfR) is downregulated, whereas expression of the ferritin heavy chain is upregulated in CT-infected HeLa-229 cells. Expression of iron-regulatory protein (IRP)-1 predominates over IRP-2 in infected cells. In infected cells, attenuated binding activity of IRP-iron responsive elements (IREs) is observed using the electrophoretic mobility-shift assay. These results suggest that iron homeostasis is modulated in CT-infected HeLa cells at the interface of acquisition and commensal use of iron.

MeSH Terms
Analysis of Variance Apoferritins/biosynthesis,genetics,metabolism Chlamydia Infections/genetics,metabolism Chlamydia trachomatis/metabolism Down-Regulation HeLa Cells Humans Iron/metabolism Iron Regulatory Protein 1/biosynthesis,genetics,metabolism Iron Regulatory Protein 2/biosynthesis,genetics,metabolism Protein Binding Receptors, Transferrin/biosynthesis,genetics,metabolism Response Elements Up-Regulation
Chemicals
Receptors, Transferrin Apoferritins Iron Iron Regulatory Protein 1 Iron Regulatory Protein 2
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Vardhan Harsh
Division of Microbiology/Tissue Culture, Institute of Pathology, Indian Council of Medical Research (ICMR), Safdarjung Hospital Campus, New Delhi, Pin 110029, India.
Bhengraj Apurb R
Jha Rajneesh
Singh Mittal Aruna
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Article Info
Journal
Journal of biomedicine & biotechnology
Abbr.
J Biomed Biotechnol
ISSN
1110-7251
DOI
10.1155/2009/342032
Published
2009-00-00
Epub
2009-00-16
Pages
342032
Language
English
Region
United States
NLM ID
101135740
PMCID
PMC2727623
Subset
IM
External Links
PubMed source DOI: 10.1155/2009/342032 PMC Full Text
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