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题目:: Establishment and characterization of an ovarian cell line of the silkworm, Bombyx mori.
作者:: Pan(Min-Hui),Cai(Xiu-Juan),Liu(Min),Lv(Jun),Tang(Hui),Tan(Juan),Lu(Cheng)
状态:: 发布时间2010-01-29 , 更新时间 2010-01-29
期刊:: Tissue Cell
摘要:: A cell line BmN-SWU1 was established from the ovarian tissues of 3-day-old fourth instar Bombyx mori larvae of the 21-872nlw variety by performing primary cultures in Grace's medium supplemented with 20% fetal bovine serum (FBS). The cell line primarily consisted of short spindle cells and round cells. The frequency of cells with chromosome number 2n=56 was 80.5%; therefore, the cell line was considered to be a diploid cell line. The population-doubling time (PDT) at 45th passage line was 57.7h. This cell line was susceptible to the B. mori nuclear polyhedrovirus (BmNPV), and the median tissue culture infective dose (TCID(50)) at a cell density of 10(5) cells/ml was 16.3 OBs/ml. The transient expression efficiency of the green fluorescent protein (GFP) gene in this cell line was 54.8%. We used the BmN-SWU1 cell line to select and establish a GFP transgenic cell line.
语言:: eng
DOI:: 10.1016/j.tice.2009.07.002

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