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PMID: 1965952 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Review

Multiple ligand binding functions for VLA-2 (alpha 2 beta 1) and VLA-3 (alpha 3 beta 1) in the integrin family.

Cell differentiation and development : the official journal of the International Society of Developmental Biologists ·Vol. 32 ·No. 3 ·1990-12-02 ·Pages 229-38

Hemler ME, Elices MJ, Chan BM, Zetter B, Matsuura N, Takada Y

Abstract

In our studies of VLA-3 we have shown (i) that a single integrin (VLA-3) can bind to multiple ligands by different mechanisms, involving RGD and non-RGD sites, which are regulated differently by divalent cations. Also we showed from the primary sequence of VLA-3 that it is only distantly related to the other cleaved alpha subunits. In our studies of VLA-2 we have shown that a single integrin may have at least three functional forms, depending on the cell type where expressed. In addition, we have expressed functional VLA-2 in RD cells, resulting in both Coll and Lm binding functions in vitro, and increased tumor cell metastasis in vivo in nude mice.

MeSH Terms

Amino Acid Sequence Animals Binding, Competitive Cations, Divalent/metabolism Cell Adhesion Collagen/metabolism Fibronectins/metabolism Humans Laminin/metabolism Ligands Mice Mice, Nude Molecular Sequence Data Neoplasm Proteins/metabolism Oligopeptides/metabolism Protein Conformation Receptors, Cell Surface/metabolism Receptors, Collagen Receptors, Fibronectin Receptors, Immunologic/metabolism Receptors, Laminin Receptors, Peptide Receptors, Very Late Antigen/metabolism Recombinant Proteins/metabolism Tumor Cells, Cultured/metabolism

Chemicals

Cations, Divalent Fibronectins Laminin Ligands Neoplasm Proteins Oligopeptides Receptors, Cell Surface Receptors, Collagen Receptors, Fibronectin Receptors, Immunologic Receptors, Laminin Receptors, Peptide Receptors, Very Late Antigen Recombinant Proteins arginyl-glycyl-aspartic acid directed cell adhesion receptor arginyl-glycyl-aspartic acid Collagen glycyl-arginyl-glycyl-aspartyl-seryl-proline glycyl-arginyl-glycyl-glutamyl-seryl-proline

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Hemler M E

Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115.

Elices M J

Chan B M

Zetter B

Matsuura N

Takada Y

Article Info

Journal

Cell differentiation and development : the official journal of the International Society of Developmental Biologists

Abbr.

Cell Differ Dev

ISSN

0922-3371

DOI

10.1016/0922-3371(90)90035-u

Published

1990-12-02

Pages

229-38

Language

English

Region

Ireland

NLM ID

8811335

Subset

Grants

NIGMS NIH HHS · GM38903 · United States

External Links

PubMed source DOI: 10.1016/0922-3371(90)90035-u

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Multiple ligand binding functions for VLA-2 (alpha 2 beta 1) and VLA-3 (alpha 3 beta 1) in the integrin family.

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