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PMID: 19648369 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Microbial biogeography of six salt lakes in Inner Mongolia, China, and a salt lake in Argentina.

Applied and environmental microbiology ·Vol. 75 ·No. 18 ·2009-09-00 ·Pages 5750-60

Pagaling E, Wang H, Venables M, Wallace A, Grant WD, Cowan DA, Jones BE, Ma Y, Ventosa A, Heaphy S

Abstract

We used cultivation-independent methods to investigate the prokaryotic biogeography of the water column in six salt lakes in Inner Mongolia, China, and a salt lake in Argentina. These lakes had different salt compositions and pH values and were at variable geographic distances, on both local and intercontinental scales, which allowed us to explore the microbial community composition within the context of both contemporary environmental conditions and geographic distance. Fourteen 16S rRNA gene clone libraries were constructed, and over 200 16S rRNA gene sequences were obtained. These sequences were used to construct biotic similarity matrices, which were used in combination with environmental similarity matrices and a distance matrix in the Mantel test to discover which factors significantly influenced biotic similarity. We showed that archaeal biogeography was influenced by contemporary environmental factors alone (Na+, CO3(2-), and HCO3(-) ion concentrations; pH; and temperature). Bacterial biogeography was influenced both by contemporary environmental factors (Na+, Mg2+, and HCO3(-) ion concentrations and pH) and by geographic distance.

MeSH Terms
Archaea/classification,genetics,isolation & purification Argentina Bacteria/classification,genetics,isolation & purification Biodiversity China Cluster Analysis DNA, Archaeal/chemistry,genetics DNA, Bacterial/chemistry,genetics DNA, Ribosomal/chemistry,genetics Genes, rRNA Hydrogen-Ion Concentration Molecular Sequence Data Phylogeny RNA, Archaeal/genetics RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Salts/analysis Sequence Analysis, DNA Sequence Homology, Nucleic Acid Water/analysis Water Microbiology
Chemicals
DNA, Archaeal DNA, Bacterial DNA, Ribosomal RNA, Archaeal RNA, Bacterial RNA, Ribosomal, 16S Salts Water
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Pagaling Eulyn
Department of Infection, Immunity, and Inflammation, University of Leicester, University Road, Leicester LE19HN, United Kingdom.
Wang Huanzhi
Venables Madeleine
Wallace Andrew
Grant William D
Cowan Don A
Jones Brian E
Ma Yanhe
Ventosa Antonio
Heaphy Shaun
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
1098-5336
DOI
10.1128/AEM.00040-09
Published
2009-09-00
Epub
2009-00-31
Pages
5750-60
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC2747855
Subset
IM
Databases
GENBANK
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External Links
PubMed source DOI: 10.1128/AEM.00040-09 PMC Full Text View journal details
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