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PMID: 19633175 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Wash functions downstream of Rho and links linear and branched actin nucleation factors.

Development (Cambridge, England) ·Vol. 136 ·No. 16 ·2009-08-00 ·Pages 2849-60

Liu R, Abreu-Blanco MT, Barry KC, Linardopoulou EV, Osborn GE, Parkhurst SM

Abstract

Wiskott-Aldrich Syndrome (WAS) family proteins are Arp2/3 activators that mediate the branched-actin network formation required for cytoskeletal remodeling, intracellular transport and cell locomotion. Wasp and Scar/WAVE, the two founding members of the family, are regulated by the GTPases Cdc42 and Rac, respectively. By contrast, linear actin nucleators, such as Spire and formins, are regulated by the GTPase Rho. We recently identified a third WAS family member, called Wash, with Arp2/3-mediated actin nucleation activity. We show that Drosophila Wash interacts genetically with Arp2/3, and also functions downstream of Rho1 with Spire and the formin Cappuccino to control actin and microtubule dynamics during Drosophila oogenesis. Wash bundles and crosslinks F-actin and microtubules, is regulated by Rho1, Spire and Arp2/3, and is essential for actin cytoskeleton organization in the egg chamber. Our results establish Wash and Rho as regulators of both linear- and branched-actin networks, and suggest an Arp2/3-mediated mechanism for how cells might coordinately regulate these structures.

MeSH Terms
Actin-Related Protein 2-3 Complex/genetics,metabolism Actins/genetics,metabolism Animals Cytoskeleton/metabolism Drosophila Proteins/genetics,metabolism Drosophila melanogaster/anatomy & histology,metabolism Female Mice Mice, Inbred BALB C Microfilament Proteins/genetics,metabolism Microtubules/metabolism Oogenesis/physiology Ovary/cytology,metabolism Vesicular Transport Proteins/genetics,metabolism Wiskott-Aldrich Syndrome Protein/genetics,metabolism rho GTP-Binding Proteins/genetics,metabolism
Chemicals
Actin-Related Protein 2-3 Complex Actins Drosophila Proteins Microfilament Proteins Vesicular Transport Proteins WASH protein, mouse Wiskott-Aldrich Syndrome Protein capu protein, Drosophila spir protein, Drosophila Rho1 protein, Drosophila rho GTP-Binding Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Liu Raymond
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
Abreu-Blanco Maria Teresa
Barry Kevin C
Linardopoulou Elena V
Osborn Gregory E
Parkhurst Susan M
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Article Info
Journal
Development (Cambridge, England)
Abbr.
Development
ISSN
1477-9129
DOI
10.1242/dev.035246
Published
2009-08-00
Pages
2849-60
Language
English
Region
England
NLM ID
8701744
PMCID
PMC2730411
Subset
IM
Grants
NIGMS NIH HHS · GM072581 · United States
External Links
PubMed source DOI: 10.1242/dev.035246 PMC Full Text View journal details
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