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PMID: 1961765 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Both activation and repression of a-mating-type-specific genes in yeast require transcription factor Mcm1.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 23 ·1991-12-01 ·Pages 10966-70

Elble R, Tye BK

Abstract

Mcm1 is a yeast transcription factor with homologs throughout the metazoa. MCM1 was first identified as a gene involved in maintenance of artificial minichromosomes in yeast. More recently Mcm1 has been shown to serve as a transcriptional regulator of mating-type-specific genes. Biochemical data suggest that Mcm1 coactivates alpha-specific genes and corepresses a-specific genes by binding to a 10-base-pair dyad symmetry element in their upstream regions. We reported previously that an mcm1 point mutation reduced activation of alpha-specific genes but had little effect on the expression of a-specific genes. We now show that another mcm1 allele, which depletes the Mcm1 protein, affects both activation and repression of a-specific genes. The mutant strain remains capable of high levels of pheromone induction of a-specific genes, although with retarded kinetics. Mcm1 joins an increasing number of transcription factors involved in both positive and negative regulation of gene expression.

Related Genes
MCM1 mcm1
MeSH Terms
Alleles Chromosome Deletion Fungal Proteins/genetics,metabolism Gene Expression Regulation, Fungal Genes, Fungal Genes, Mating Type, Fungal Kinetics Mating Factor Minichromosome Maintenance 1 Protein Mutagenesis, Insertional Peptides/genetics Pheromones/genetics Plasmids Restriction Mapping Saccharomyces cerevisiae/genetics Transcription Factors/genetics,metabolism Transcription, Genetic Transcriptional Activation beta-Galactosidase/genetics,metabolism
Chemicals
Fungal Proteins Minichromosome Maintenance 1 Protein Peptides Pheromones Transcription Factors Mating Factor beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Elble R
Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853.
Tye B K
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.23.10966
Published
1991-12-01
Pages
10966-70
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53053
Subset
IM
Grants
NIGMS NIH HHS · GM34190 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.23.10966 PMC Full Text View journal details
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