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PMID: 1961738 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Mouse kappa light-chain recombination signal sequences mediate recombination more frequently than do those of lambda light chain.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 23 ·1991-12-01 ·Pages 10721-5

Ramsden DA, Wu GE

Abstract

Immunoglobulin and T-cell receptor genes are somatically rearranged by site-specific recombination. Recombination signal sequences (RSS) have been identified as the major targeting element of this process. Recent reports demonstrate that differences in RSS affect the frequency of recombination, suggesting a role for RSS in the development of the B-cell repertoire. Examination of mouse light-chain RSS indicates that kappa light-chain RSS consistently show a greater degree of similarity to a consensus sequence than do those of lambda light chain. To determine whether this difference in natural RSS could affect the patterns of light-chain gene rearrangement and expression, we have constructed recombination substrates containing both a typical mouse kappa RSS pair and a typical mouse lambda RSS pair. Experiments using these substrates demonstrate that the kappa RSS pair mediates recombination at a vastly higher frequency than does the lambda RSS pair. This result argues that RSS differences may contribute significantly to the patterns of mouse immunoglobulin light-chain rearrangement, ultimately resulting in a high proportion of kappa light chain relative to lambda.

MeSH Terms
Animals Base Sequence Cell Line DNA Nucleotidyltransferases/metabolism Gene Rearrangement Immunoglobulin Light Chains/genetics Immunoglobulin kappa-Chains/genetics Immunoglobulin lambda-Chains/genetics Mice Molecular Sequence Data Oligodeoxyribonucleotides Plasmids Protein Sorting Signals/genetics Recombination, Genetic Sequence Homology, Nucleic Acid VDJ Recombinases
Chemicals
Immunoglobulin Light Chains Immunoglobulin kappa-Chains Immunoglobulin lambda-Chains Oligodeoxyribonucleotides Protein Sorting Signals DNA Nucleotidyltransferases VDJ Recombinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ramsden D A
Department of Immunology, University of Toronto, Ontario, Canada.
Wu G E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.23.10721
Published
1991-12-01
Pages
10721-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53002
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.88.23.10721 PMC Full Text View journal details
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