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PMID: 19571877 Published · ppublish English Journal Article

Elite and stochastic models for induced pluripotent stem cell generation.

Nature ·Vol. 460 ·No. 7251 ·2009-07-02 ·Pages 49-52

Yamanaka S

Abstract

Induced pluripotent stem cells offer unprecedented potential for disease research, drug screening, toxicology and regenerative medicine. However, the process of reprogramming is inefficient and often incomplete. Here I consider reasons for bottlenecks in induced pluripotent stem cell generation, and propose a model in which most or all cells have the potential to become pluripotent.

MeSH Terms
Animals Cell Differentiation Cell Lineage Cellular Reprogramming/genetics Epigenesis, Genetic Humans Mice Models, Biological Pluripotent Stem Cells/cytology,metabolism Stochastic Processes Transduction, Genetic
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Yamanaka Shinya
Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan. yamanaka@cira.kyoto-u.ac.jp
References (39)
39 references, click to expand
  1. c-Myc is dispensable for direct reprogramming of mouse fibroblasts.
    Cell Stem Cell. 2008 Jan 10;2(1):10-2 PMID: 18371415
  2. A drug-inducible transgenic system for direct reprogramming of multiple somatic cell types.
    Nat Biotechnol. 2008 Aug;26(8):916-24 PMID: 18594521
  3. Oct4-induced pluripotency in adult neural stem cells.
    Cell. 2009 Feb 6;136(3):411-9 PMID: 19203577
  4. Transcriptional repression and DNA hypermethylation of a small set of ES cell marker genes in male germline stem cells.
    BMC Dev Biol. 2006 Jul 21;6:34 PMID: 16859545
  5. Reprogramming of pancreatic beta cells into induced pluripotent stem cells.
    Curr Biol. 2008 Jun 24;18(12):890-4 PMID: 18501604
  6. Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes.
    Nat Biotechnol. 2008 Nov;26(11):1276-84 PMID: 18931654
  7. A bivalent chromatin structure marks key developmental genes in embryonic stem cells.
    Cell. 2006 Apr 21;125(2):315-26 PMID: 16630819
  8. Generation of pluripotent stem cells from adult mouse liver and stomach cells.
    Science. 2008 Aug 1;321(5889):699-702 PMID: 18276851
  9. Induced pluripotent stem cells generated without viral integration.
    Science. 2008 Nov 7;322(5903):945-9 PMID: 18818365
  10. Induced pluripotent stem cell lines derived from human somatic cells.
    Science. 2007 Dec 21;318(5858):1917-20 PMID: 18029452
  11. piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells.
    Nature. 2009 Apr 9;458(7239):766-70 PMID: 19252478
  12. Generation of germline-competent induced pluripotent stem cells.
    Nature. 2007 Jul 19;448(7151):313-7 PMID: 17554338
  13. Reprogramming efficiency following somatic cell nuclear transfer is influenced by the differentiation and methylation state of the donor nucleus.
    Stem Cells. 2006 Sep;24(9):2007-13 PMID: 16709876
  14. Reprogramming of human somatic cells to pluripotency with defined factors.
    Nature. 2008 Jan 10;451(7175):141-6 PMID: 18157115
  15. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
    Cell. 2006 Aug 25;126(4):663-76 PMID: 16904174
  16. Isolation of multipotent adult stem cells from the dermis of mammalian skin.
    Nat Cell Biol. 2001 Sep;3(9):778-84 PMID: 11533656
  17. Human induced pluripotent stem cells free of vector and transgene sequences.
    Science. 2009 May 8;324(5928):797-801 PMID: 19325077
  18. Directly reprogrammed fibroblasts show global epigenetic remodeling and widespread tissue contribution.
    Cell Stem Cell. 2007 Jun 7;1(1):55-70 PMID: 18371336
  19. Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts.
    Nat Biotechnol. 2008 Jan;26(1):101-6 PMID: 18059259
  20. In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state.
    Nature. 2007 Jul 19;448(7151):318-24 PMID: 17554336
  21. Fibroblast-derived induced pluripotent stem cells show no common retroviral vector insertions.
    Stem Cells. 2009 Feb;27(2):300-6 PMID: 19008347
  22. Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse.
    Cell Stem Cell. 2008 Mar 6;2(3):230-40 PMID: 18371448
  23. KLF4, p21 and context-dependent opposing forces in cancer.
    Nat Rev Cancer. 2006 Jan;6(1):11-23 PMID: 16372018
  24. Generation of human induced pluripotent stem cells from dermal fibroblasts.
    Proc Natl Acad Sci U S A. 2008 Feb 26;105(8):2883-8 PMID: 18287077
  25. Reprogramming of neural progenitor cells into induced pluripotent stem cells in the absence of exogenous Sox2 expression.
    Stem Cells. 2008 Oct;26(10):2467-74 PMID: 18635867
  26. Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2.
    Nat Biotechnol. 2008 Nov;26(11):1269-75 PMID: 18849973
  27. Generation of induced pluripotent stem cells using recombinant proteins.
    Cell Stem Cell. 2009 May 8;4(5):381-4 PMID: 19398399
  28. Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells.
    Cell Stem Cell. 2008 Feb 7;2(2):151-9 PMID: 18371436
  29. Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds.
    Nat Biotechnol. 2008 Jul;26(7):795-7 PMID: 18568017
  30. A high-efficiency system for the generation and study of human induced pluripotent stem cells.
    Cell Stem Cell. 2008 Sep 11;3(3):340-5 PMID: 18786420
  31. Myc influences global chromatin structure.
    EMBO J. 2006 Jun 21;25(12):2723-34 PMID: 16724113
  32. Promotion of reprogramming to ground state pluripotency by signal inhibition.
    PLoS Biol. 2008 Oct 21;6(10):e253 PMID: 18942890
  33. Fbx15 is a novel target of Oct3/4 but is dispensable for embryonic stem cell self-renewal and mouse development.
    Mol Cell Biol. 2003 Apr;23(8):2699-708 PMID: 12665572
  34. Generation of mouse induced pluripotent stem cells without viral vectors.
    Science. 2008 Nov 7;322(5903):949-53 PMID: 18845712
  35. Direct reprogramming of terminally differentiated mature B lymphocytes to pluripotency.
    Cell. 2008 Apr 18;133(2):250-64 PMID: 18423197
  36. Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells.
    Nat Genet. 2000 Apr;24(4):372-6 PMID: 10742100
  37. Induction of pluripotent stem cells from adult human fibroblasts by defined factors.
    Cell. 2007 Nov 30;131(5):861-72 PMID: 18035408
  38. Dissecting direct reprogramming through integrative genomic analysis.
    Nature. 2008 Jul 3;454(7200):49-55 PMID: 18509334
  39. Small increases in the level of Sox2 trigger the differentiation of mouse embryonic stem cells.
    Stem Cells. 2008 Apr;26(4):903-11 PMID: 18238855
Article Info
Journal
Nature
Abbr.
Nature
ISSN
1476-4687
DOI
10.1038/nature08180
Email
yamanaka@cira.kyoto-u.ac.jp
Published
2009-07-02
Pages
49-52
Language
English
Region
England
NLM ID
0410462
Subset
IM
External Links
PubMed source DOI: 10.1038/nature08180 View journal details
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