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PMID: 19558960 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Identification of a multidrug efflux pump in Flavobacterium johnsoniae.

Veterinary research ·Vol. 40 ·No. 6 ·2009-00-00 ·Pages 55

Clark SE, Jude BA, Danner GR, Fekete FA

Abstract

In this study, the mechanism conferring multiple drug resistance in several strains of flavobacteria isolated from the ovarian fluids of hatchery reared 3-year old brook trout Salvelinus fontinalis was investigated. Metabolic fingerprinting and 16S rRNA gene sequences identified the isolates as Flavobacterium johnsoniae. The isolates exhibited multiple resistances to a wide range of antimicrobial classes including penicillin, cephem, monobactam, aminoglycoside, and phenicol. Although plasmids and other transposable elements containing antimicrobial resistance genes were not detected, the isolates did contain a genomic sequence for a chloramphenicol-inducible resistance-nodulation-division family multidrug efflux pump system. Efflux pumps are non-specific multidrug efflux systems. They are also a component of cell-cell communication systems, and respond specifically to cell membrane stressors such as oxidative or nitrosative stress. Understanding of efflux pump mediated antibiotic resistances will affect efficacy of clinical treatments of fishes associated with F. johnsoniae epizootics.

MeSH Terms
Amino Acid Sequence Anti-Bacterial Agents/metabolism Bacterial Proteins/genetics,metabolism Cloning, Molecular Drug Resistance, Multiple, Bacterial/genetics Flavobacterium/drug effects,metabolism Gene Expression Regulation, Bacterial Membrane Transport Proteins/metabolism Molecular Sequence Data
Chemicals
Anti-Bacterial Agents Bacterial Proteins Membrane Transport Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Clark Sarah E
Department of Biology, Colby College, 5729 Mayflower Hill Drive, Waterville, ME 04901, USA.
Jude Brooke A
Danner G Russell
Fekete Frank A
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Article Info
Journal
Veterinary research
Abbr.
Vet Res
ISSN
0928-4249
DOI
10.1051/vetres/2009038
Published
2009-00-00
Epub
2009-00-27
Pages
55
Language
English
Region
England
NLM ID
9309551
PMCID
PMC2717357
Subset
IM
Grants
NCRR NIH HHS · P20 RR016463 · United States
NCRR NIH HHS · P20 RR16463 · United States
External Links
PubMed source DOI: 10.1051/vetres/2009038 PMC Full Text View journal details
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