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PMID: 19542444 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

The initial phase of an immune response functions to activate regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950) ·Vol. 183 ·No. 1 ·2009-07-01 ·Pages 332-9

O'Gorman WE, Dooms H, Thorne SH, Kuswanto WF, Simonds EF, Krutzik PO, Nolan GP, Abbas AK

Abstract

An early reaction of CD4(+) T lymphocytes to Ag is the production of cytokines, notably IL-2. To detect cytokine-dependent responses, naive Ag-specific T cells were stimulated in vivo and the presence of phosphorylated STAT5 molecules was used to identify the cell populations responding to IL-2. Within hours of T cell priming, IL-2-dependent STAT5 phosphorylation occurred primarily in Foxp3(+) regulatory T cells. In contrast, the Ag-specific T cells received STAT5 signals only after repeated Ag exposure or memory differentiation. Regulatory T cells receiving IL-2 signals proliferated and developed enhanced suppressive activity. These results indicate that one of the earliest events in a T cell response is the activation of endogenous regulatory cells, potentially to prevent autoimmunity.

MeSH Terms
Animals Cell Proliferation Interleukin-2/biosynthesis,physiology Lymphocyte Activation/genetics,immunology Mice Mice, Inbred BALB C Mice, Knockout Mice, Transgenic Phosphorylation Resting Phase, Cell Cycle/genetics,immunology STAT5 Transcription Factor/metabolism Signal Transduction/genetics,immunology T-Lymphocyte Subsets/immunology,metabolism,virology T-Lymphocytes, Regulatory/immunology,metabolism,virology Time Factors Vaccinia virus/immunology
Chemicals
Interleukin-2 STAT5 Transcription Factor
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
O'Gorman William E
Department of Microbiology and Immunology, Stanford University School of Medicine, CA 94305, USA.
Dooms Hans
Thorne Steve H
Kuswanto Wilson F
Simonds Erin F
Krutzik Peter O
Nolan Garry P
Abbas Abul K
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Article Info
Journal
Journal of immunology (Baltimore, Md. : 1950)
Abbr.
J Immunol
ISSN
1550-6606
DOI
10.4049/jimmunol.0900691
Published
2009-07-01
Pages
332-9
Language
English
Region
United States
NLM ID
2985117R
PMCID
PMC2753472
Subset
IM
Grants
NIAID NIH HHS · P01 AI035297 · United States
NIAID NIH HHS · P01 AI036535 · United States
NHLBI NIH HHS · N01-HV-28183 · United States
NIAID NIH HHS · R01 AI065824 · United States
NIAID NIH HHS · R01 AI073656 · United States
NIAID NIH HHS · P01 AI35297 · United States
NIAID NIH HHS · R01 AI073656-01A1 · United States
NHLBI NIH HHS · N01HV28183 · United States
NIAID NIH HHS · P01 AI035297-150001 · United States
NHLBI NIH HHS · N01 HV028183 · United States
NIAID NIH HHS · P01 AI036535-080006 · United States
NIAID NIH HHS · P01 AI36535 · United States
External Links
PubMed source DOI: 10.4049/jimmunol.0900691 PMC Full Text View journal details
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