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PMID: 19499023 Published · ppublish English Journal Article

Analytical ultracentrifugation as a contemporary biomolecular research tool.

Journal of biomolecular techniques : JBT ·Vol. 10 ·No. 4 ·1999-12-00 ·Pages 163-76

Cole JL, Hansen JC

Abstract

Analytical ultracentrifugation has again become a widely used biomolecular research technique for determining sample purity, characterizing assembly and disassembly mechanisms of biomolecular complexes, determining subunit stoichiometries, detecting and characterizing macromolecular conformational changes, and measuring equilibrium constants and thermodynamic parameters for self- and hetero-associating systems. Concomitant with the availability of modern instrumentation is a strong need for biomedical scientists to become acquainted with the fundamental principles of analytical ultracentrifugation and the new data analysis methodologies that have greatly transformed this technique as it exists today.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cole J L
Department of Antiviral Research, Merck Research Laboratories, West Point, PA 19486, USA.
Hansen J C
References (72)
72 references, click to expand
  1. Quantitative analysis of macromolecular conformational changes using agarose gel electrophoresis: application to chromatin folding.
    Biochemistry. 1994 Sep 13;33(36):10859-63 PMID: 8086402
  2. Boundary analysis in sedimentation transport experiments: a procedure for obtaining sedimentation coefficient distributions using the time derivative of the concentration profile.
    Anal Biochem. 1992 Jun;203(2):295-301 PMID: 1416025
  3. Direct analysis of sedimentation equilibrium distributions reflecting complex formation between dissimilar reactants.
    Biochemistry. 1998 Feb 24;37(8):2226-33 PMID: 9485368
  4. Structure-function studies on small heat shock protein oligomeric assembly and interaction with unfolded polypeptides.
    J Biol Chem. 1997 Sep 26;272(39):24646-56 PMID: 9305934
  5. Linkage between operator binding and dimer to octamer self-assembly of bacteriophage lambda cI repressor.
    Biochemistry. 1997 Oct 21;36(42):12994-3003 PMID: 9335560
  6. Biochemical, biophysical, and pharmacological characterization of bacterially expressed human agouti-related protein.
    Biochemistry. 1998 Nov 17;37(46):16041-52 PMID: 9819197
  7. Type II DNA topoisomerase from Saccharomyces cerevisiae is a stable dimer.
    Biochemistry. 1997 May 20;36(20):6107-14 PMID: 9166781
  8. Molecular mass determination by sedimentation velocity experiments and direct fitting of the concentration profiles.
    Biophys J. 1997 Jan;72(1):428-34 PMID: 8994629
  9. Interaction of the low-affinity receptor CD23/Fc epsilonRII lectin domain with the Fc epsilon3-4 fragment of human immunoglobulin E.
    Biochemistry. 1997 Feb 25;36(8):2112-22 PMID: 9047310
  10. De novo design of a model peptide sequence to examine the effects of single amino acid substitutions in the hydrophobic core on both stability and oligomerization state of coiled-coils.
    J Mol Biol. 1999 Jan 15;285(2):785-803 PMID: 9878444
  11. Activation of RNase L by 2',5'-oligoadenylates. Biophysical characterization.
    J Biol Chem. 1997 Aug 1;272(31):19187-92 PMID: 9235909
  12. Rapid determination of molecular weights of peptides and preteins.
    Ann N Y Acad Sci. 1960 Aug 31;88:586-601 PMID: 13787531
  13. The cyanobacterial repressor SmtB is predominantly a dimer and binds two Zn2+ ions per subunit.
    Biochemistry. 1997 Dec 9;36(49):15343-8 PMID: 9398263
  14. Biophysical characterization of a designed TMV coat protein mutant, R46G, that elicits a moderate hypersensitivity response in Nicotiana sylvestris.
    Protein Sci. 1999 Feb;8(2):261-70 PMID: 10048319
  15. Parameter estimation by least-squares methods.
    Methods Enzymol. 1992;210:1-37 PMID: 1584035
  16. Sensitivity of Drosophila heat shock transcription factor to low pH.
    J Biol Chem. 1999 Jan 29;274(5):3135-40 PMID: 9915852
  17. Rapid and accurate microfractionation of the contents of small centrifuge tubes: application in the measurement of molecular weight of proteins via sedimentation equilibrium.
    Anal Biochem. 1993 Feb 15;209(1):130-5 PMID: 8465945
  18. Linker histones stabilize the intrinsic salt-dependent folding of nucleosomal arrays: mechanistic ramifications for higher-order chromatin folding.
    Biochemistry. 1998 Oct 20;37(42):14776-87 PMID: 9778352
  19. A revised model for the oligomeric state of the N-ethylmaleimide-sensitive fusion protein, NSF.
    J Biol Chem. 1998 Jun 19;273(25):15675-81 PMID: 9624162
  20. Enhanced transcription factor access to arrays of histone H3/H4 tetramer.DNA complexes in vitro: implications for replication and transcription.
    Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12169-73 PMID: 9770458
  21. Analysis of data from the analytical ultracentrifuge by nonlinear least-squares techniques.
    Biophys J. 1981 Dec;36(3):575-88 PMID: 7326325
  22. A comparison of thermodynamic parameters for vinorelbine- and vinflunine-induced tubulin self-association by sedimentation velocity.
    Mol Pharmacol. 1998 May;53(5):908-15 PMID: 9584218
  23. Dissociation equilibria of the tryptophan synthase alpha 2 beta 2 complex in saline buffer and guanidine isothiocyanate, as studied by sedimentation equilibrium.
    Biophys Chem. 1997 Nov;69(1):53-62 PMID: 9440208
  24. Comparison of the salt-dependent self-association of brain and erythroid spectrin.
    Biochemistry. 1997 Jun 10;36(23):6977-85 PMID: 9188694
  25. Phage phi29 protein p6 is in a monomer-dimer equilibrium that shifts to higher association states at the millimolar concentrations found in vivo.
    Biochemistry. 1997 Sep 30;36(39):11901-8 PMID: 9305983
  26. Binding of pyridoxal 5'-phosphate to the heme protein human cystathionine beta-synthase.
    Biochemistry. 1999 Mar 2;38(9):2716-24 PMID: 10052942
  27. Disruption of higher-order folding by core histone acetylation dramatically enhances transcription of nucleosomal arrays by RNA polymerase III.
    Mol Cell Biol. 1998 Aug;18(8):4629-38 PMID: 9671473
  28. Pyruvate dehydrogenase multienzyme complex. Characterization of assembly intermediates by sedimentation velocity analysis.
    J Biol Chem. 1994 Dec 16;269(50):31372-7 PMID: 7989301
  29. Reversible oligonucleosome self-association: dependence on divalent cations and core histone tail domains.
    Biochemistry. 1996 Apr 2;35(13):4009-15 PMID: 8672434
  30. Association of terminal complement proteins in solution and modulation by suramin.
    Biochemistry. 1999 May 25;38(21):6807-16 PMID: 10346902
  31. cAMP response element-binding protein monomers cooperatively assemble to form dimers on DNA.
    J Biol Chem. 1998 Aug 14;273(33):20820-7 PMID: 9694827
  32. Heparin is essential for a single keratinocyte growth factor molecule to bind and form a complex with two molecules of the extracellular domain of its receptor.
    Biochemistry. 1999 Feb 23;38(8):2523-34 PMID: 10029547
  33. The direct analysis of sedimentation equilibrium results obtained with polymerizing systems.
    Biophys Chem. 1975 Apr;3(2):169-76 PMID: 1148373
  34. Sedimentation analysis of noninteracting and self-associating solutes using numerical solutions to the Lamm equation.
    Biophys J. 1998 Sep;75(3):1503-12 PMID: 9726952
  35. The self-association of biglycan from bovine articular cartilage.
    J Biol Chem. 1994 Nov 11;269(45):28366-73 PMID: 7961776
  36. Domain structure of the Staphylococcus aureus collagen adhesin.
    Biochemistry. 1998 Nov 3;37(44):15423-33 PMID: 9799504
  37. Construction of hydrodynamic bead models from high-resolution X-ray crystallographic or nuclear magnetic resonance data.
    Biophys J. 1997 Jan;72(1):408-15 PMID: 8994627
  38. Structural and biological stability of the human interleukin 10 homodimer.
    Biochemistry. 1998 Dec 1;37(48):16943-51 PMID: 9836587
  39. Detergent-solubilized monomeric and dimeric cytochrome bc1 isolated from bovine heart.
    Biochemistry. 1994 Nov 8;33(44):13005-12 PMID: 7947705
  40. Defining the structure and stability of macromolecular assemblies in solution: the re-emergence of analytical ultracentrifugation as a practical tool.
    Structure. 1996 Apr 15;4(4):367-73 PMID: 8740359
  41. An approximate solution to the Lamm equation.
    Biophys Chem. 1979 Sep;10(2):187-90 PMID: 16997215
  42. Core histone tail domains mediate oligonucleosome folding and nucleosomal DNA organization through distinct molecular mechanisms.
    J Biol Chem. 1995 Oct 27;270(43):25359-62 PMID: 7592700
  43. Determination of molecular parameters by fitting sedimentation data to finite-element solutions of the Lamm equation.
    Biophys J. 1998 Jan;74(1):444-54 PMID: 9449345
  44. The uvsY recombination protein of bacteriophage T4 forms hexamers in the presence and absence of single-stranded DNA.
    Biochemistry. 1998 Apr 21;37(16):5673-81 PMID: 9548953
  45. An improved function for fitting sedimentation velocity data for low-molecular-weight solutes.
    Biophys J. 1997 Jan;72(1):435-44 PMID: 8994630
  46. HYDRO: a computer program for the prediction of hydrodynamic properties of macromolecules.
    Biophys J. 1994 Aug;67(2):530-1 PMID: 7948671
  47. Modern applications of analytical ultracentrifugation.
    Annu Rev Biophys Biomol Struct. 1999;28:75-100 PMID: 10410796
  48. Effect of phospholipase A2 digestion on the conformation and lysine/fibrinogen binding properties of human lipoprotein[a].
    J Lipid Res. 1999 Apr;40(4):583-92 PMID: 10191282
  49. Characterization of human cytomegalovirus protease dimerization by analytical centrifugation.
    Biochemistry. 1996 Dec 3;35(48):15601-10 PMID: 8952514
  50. The core histone N-terminal domains are required for multiple rounds of catalytic chromatin remodeling by the SWI/SNF and RSC complexes.
    Biochemistry. 1999 Feb 23;38(8):2514-22 PMID: 10029546
  51. Boundary analysis in sedimentation velocity experiments.
    Methods Enzymol. 1994;240:478-501 PMID: 7823845
  52. Bacteriophage P22 scaffolding protein forms oligomers in solution.
    J Mol Biol. 1997 May 9;268(3):655-65 PMID: 9171289
  53. Studying low-density lipoprotein-monoclonal antibody complexes using dynamic laser light scattering and analytical ultracentrifugation.
    Biochemistry. 1999 Jan 26;38(4):1284-92 PMID: 9930989
  54. Analytical ultracentrifugation of complex macromolecular systems.
    Biochemistry. 1994 Nov 15;33(45):13155-63 PMID: 7947722
  55. Quantitative agarose gel electrophoresis of chromatin: nucleosome-dependent changes in charge, sharp, and deformability at low ionic strength.
    Biochemistry. 1994 Mar 1;33(8):2226-33 PMID: 8117680
  56. Association states of the transcription activator protein NtrC from E. coli determined by analytical ultracentrifugation.
    J Mol Biol. 1998 May 22;278(5):915-33 PMID: 9600853
  57. Analytical centrifugation with preparative ultracentrifuges.
    Anal Biochem. 1989 Feb 1;176(2):209-16 PMID: 2662806
  58. Alteration of the quaternary structure of cpn60 modulates chaperonin-assisted folding. Implications for the mechanism of chaperonin action.
    J Biol Chem. 1994 Jan 28;269(4):2447-51 PMID: 7905478
  59. Sedimentation velocity spins a new weave for an old fabric.
    Curr Opin Biotechnol. 1997 Feb 1;8(1):14-24 PMID: 9013661
  60. Hybrid trypsinized nucleosomal arrays: identification of multiple functional roles of the H2A/H2B and H3/H4 N-termini in chromatin fiber compaction.
    Biochemistry. 1997 Sep 23;36(38):11381-8 PMID: 9298957
  61. The lipid-free structure of apolipoprotein A-I: effects of amino-terminal deletions.
    Biochemistry. 1998 Aug 25;37(34):11714-25 PMID: 9718294
  62. Identification and interpretation of complexity in sedimentation velocity boundaries.
    Biophys J. 1997 Jan;72(1):397-407 PMID: 8994626
  63. The TATA-binding protein from Saccharomyces cerevisiae oligomerizes in solution at micromolar concentrations to form tetramers and octamers.
    J Mol Biol. 1999 Jan 29;285(4):1389-99 PMID: 9917384
  64. The b and delta subunits of the Escherichia coli ATP synthase interact via residues in their C-terminal regions.
    J Biol Chem. 1998 Jun 12;273(24):15162-8 PMID: 9614129
  65. Plasmid pIP501 encoded transcriptional repressor CopR binds to its target DNA as a dimer.
    J Mol Biol. 1998 Oct 30;283(3):595-603 PMID: 9784369
  66. Simultaneous rapid estimation of sedimentation coefficient and molecular weight.
    Biophys Chem. 1980 Apr;11(2):303-8 PMID: 16997250
  67. Role of guanine nucleotides in the vinblastine-induced self-association of tubulin: effects of guanosine alpha,beta-methylenetriphosphate and guanosine alpha,beta-methylenediphosphate.
    Biochemistry. 1997 Oct 21;36(42):12828-35 PMID: 9335540
  68. Hydrodynamic studies on the manganese-stabilizing protein of photosystem II.
    Biochemistry. 1998 Sep 29;37(39):13553-8 PMID: 9753441
  69. New revolutions in the evolution of analytical ultracentrifugation.
    Curr Opin Struct Biol. 1996 Oct;6(5):650-8 PMID: 8913688
  70. Listening to the customer: implementing a patient satisfaction measurement system.
    Gastroenterol Nurs. 1994 Nov-Dec;17(3):110-5 PMID: 7858003
  71. Biophysical properties of human erythrocyte spectrin at alkaline pH: implications for spectrin structure, function, and association.
    Biochemistry. 1998 Jan 6;37(1):264-71 PMID: 9425047
  72. Mechanism of Rab geranylgeranylation: formation of the catalytic ternary complex.
    Biochemistry. 1998 Sep 8;37(36):12559-68 PMID: 9730828
Article Info
Journal
Journal of biomolecular techniques : JBT
Abbr.
J Biomol Tech
ISSN
1524-0215
Published
1999-12-00
Pages
163-76
Language
English
Region
United States
NLM ID
100888641
PMCID
PMC2291609
External Links
PubMed source PMC Full Text
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