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PMID: 19492039 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

Unc5B interacts with FLRT3 and Rnd1 to modulate cell adhesion in Xenopus embryos.

PloS one ·Vol. 4 ·No. 5 ·2009-05-29 ·Pages e5742

Karaulanov E, Böttcher RT, Stannek P, Wu W, Rau M, Ogata S, Cho KW, Niehrs C

Abstract

The FLRT family of transmembrane proteins has been implicated in the regulation of FGF signalling, neurite outgrowth, homotypic cell sorting and cadherin-mediated adhesion. In an expression screen we identified the Netrin receptors Unc5B and Unc5D as high-affinity FLRT3 interactors. Upon overexpression, Unc5B phenocopies FLRT3 and both proteins synergize in inducing cell deadhesion in Xenopus embryos. Morpholino knock-downs of Unc5B and FLRT3 synergistically affect Xenopus development and induce morphogenetic defects. The small GTPase Rnd1, which transmits FLRT3 deadhesion activity, physically and functionally interacts with Unc5B, and mediates its effect on cell adhesion. The results suggest that FLRT3, Unc5B and Rnd1 proteins interact to modulate cell adhesion in early Xenopus development.

MeSH Terms

Animals Biomarkers/metabolism Cell Adhesion Embryo, Nonmammalian/cytology,metabolism Gene Expression Regulation, Developmental Membrane Proteins/genetics,metabolism Morphogenesis Netrin Receptors Protein Binding Receptors, Cell Surface/genetics,metabolism Xenopus/embryology,metabolism Xenopus Proteins/genetics,metabolism rho GTP-Binding Proteins/metabolism

Chemicals

Biomarkers FLRT3 protein, Xenopus Membrane Proteins Netrin Receptors Receptors, Cell Surface Rnd1 protein, Xenopus Xenopus Proteins rho GTP-Binding Proteins

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Karaulanov Emil

Division of Molecular Embryology, DKFZ-ZMBH Alliance, German Cancer Research Center, Heidelberg, Germany.

Böttcher Ralph T

Stannek Peter

Wu Wei

Rau Marlene

Ogata Souichi

Cho Ken W Y

Niehrs Christof

References (24)

24 references, click to expand

UNC-5, a transmembrane protein with immunoglobulin and thrombospondin type 1 domains, guides cell and pioneer axon migrations in C. elegans.
Cell. 1992 Oct 16;71(2):289-99 PMID: 1384987
TGF-beta signaling-mediated morphogenesis: modulation of cell adhesion via cadherin endocytosis.
Genes Dev. 2007 Jul 15;21(14):1817-31 PMID: 17639085
FLRT3 is expressed in sensory neurons after peripheral nerve injury and regulates neurite outgrowth.
Mol Cell Neurosci. 2004 Oct;27(2):202-14 PMID: 15485775
Gastrulation in zebrafish -- all just about adhesion?
Curr Opin Genet Dev. 2006 Aug;16(4):433-41 PMID: 16797963
The netrin receptor UNC5B mediates guidance events controlling morphogenesis of the vascular system.
Nature. 2004 Nov 11;432(7014):179-86 PMID: 15510105
A ligand-gated association between cytoplasmic domains of UNC5 and DCC family receptors converts netrin-induced growth cone attraction to repulsion.
Cell. 1999 Jun 25;97(7):927-41 PMID: 10399920
Kremen proteins are Dickkopf receptors that regulate Wnt/beta-catenin signalling.
Nature. 2002 Jun 6;417(6889):664-7 PMID: 12050670
A new member of the Rho family, Rnd1, promotes disassembly of actin filament structures and loss of cell adhesion.
J Cell Biol. 1998 Apr 6;141(1):187-97 PMID: 9531558
Ventral closure, headfold fusion and definitive endoderm migration defects in mouse embryos lacking the fibronectin leucine-rich transmembrane protein FLRT3.
Dev Biol. 2008 Jun 1;318(1):184-93 PMID: 18448090
Genetic ablation of FLRT3 reveals a novel morphogenetic function for the anterior visceral endoderm in suppressing mesoderm differentiation.
Genes Dev. 2008 Dec 1;22(23):3349-62 PMID: 19056886
Netrin-1 acts as a survival factor via its receptors UNC5H and DCC.
EMBO J. 2001 Jun 1;20(11):2715-22 PMID: 11387206
A role for fibronectin-leucine-rich transmembrane cell-surface proteins in homotypic cell adhesion.
EMBO Rep. 2006 Mar;7(3):283-90 PMID: 16440004
Regulated adhesion as a driving force of gastrulation movements.
Development. 2008 Nov;135(22):3625-41 PMID: 18952908
The netrin receptor UNC5B promotes angiogenesis in specific vascular beds.
Development. 2008 Feb;135(4):659-67 PMID: 18223200
Rnd1, a novel rho family GTPase, induces the formation of neuritic processes in PC12 cells.
Biochem Biophys Res Commun. 2000 Nov 30;278(3):604-8 PMID: 11095956
Function and regulation of Rnd proteins.
Nat Rev Mol Cell Biol. 2006 Jan;7(1):54-62 PMID: 16493413
Identification of FLRT1, FLRT2, and FLRT3: a novel family of transmembrane leucine-rich repeat proteins.
Genomics. 1999 Dec 15;62(3):417-26 PMID: 10644439
p53, apoptosis and axon-guidance molecules.
Cell Death Differ. 2005 Aug;12(8):1057-65 PMID: 15818407
FLRT3, a cell surface molecule containing LRR repeats and a FNIII domain, promotes neurite outgrowth.
Biochem Biophys Res Commun. 2004 Jan 23;313(4):1086-91 PMID: 14706654
Xenopus cadherins: sorting out types and functions in embryogenesis.
Dev Dyn. 1996 Oct;207(2):121-34 PMID: 8906416
Regulation of cadherin-mediated adhesion in morphogenesis.
Nat Rev Mol Cell Biol. 2005 Aug;6(8):622-34 PMID: 16025097
Vertebrate homologues of C. elegans UNC-5 are candidate netrin receptors.
Nature. 1997 Apr 24;386(6627):833-8 PMID: 9126742
The transmembrane protein XFLRT3 forms a complex with FGF receptors and promotes FGF signalling.
Nat Cell Biol. 2004 Jan;6(1):38-44 PMID: 14688794
Quantitative assessment of the knockdown efficiency of morpholino antisense oligonucleotides in zebrafish embryos using a luciferase assay.
Genesis. 2008 Jan;46(1):1-7 PMID: 18196596

Article Info

Journal

PloS one

Abbr.

PLoS One

ISSN

1932-6203

DOI

10.1371/journal.pone.0005742

Published

2009-05-29

Epub

2009-00-29

Pages

e5742

Language

English

Region

United States

NLM ID

101285081

PMCID

PMC2683942

Subset

External Links

PubMed source DOI: 10.1371/journal.pone.0005742 PMC Full Text View journal details

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Unc5B interacts with FLRT3 and Rnd1 to modulate cell adhesion in Xenopus embryos.

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