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PMID: 19490898 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

RhoBTB3: a Rho GTPase-family ATPase required for endosome to Golgi transport.

Cell ·Vol. 137 ·No. 5 ·2009-05-29 ·Pages 938-48

Espinosa EJ, Calero M, Sridevi K, Pfeffer SR

Abstract

Rho GTPases are key regulators of the actin-based cytoskeleton; Rab GTPases are key regulators of membrane traffic. We report here that the atypical Rho GTPase family member, RhoBTB3, binds directly to Rab9 GTPase and functions with Rab9 in protein transport from endosomes to the trans Golgi network. Gene replacement experiments show that RhoBTB3 function in cultured cells requires both RhoBTB3's N-terminal, Rho-related domain and C-terminal sequences that are important for Rab9 interaction. Biochemical analysis reveals that RhoBTB3 binds and hydrolyzes ATP rather than GTP. Rab9 binding opens the autoinhibited RhoBTB3 protein to permit maximal ATP hydrolysis. Because RhoBTB3 interacts with TIP47 on membranes, we propose that it may function to release this cargo selection protein from vesicles to permit their efficient docking and fusion at the Golgi.

MeSH Terms
Adenosine Triphosphate/metabolism Amino Acid Sequence DNA-Binding Proteins/metabolism Endosomes/metabolism Golgi Apparatus/metabolism Humans Intracellular Signaling Peptides and Proteins/metabolism Molecular Sequence Data Perilipin-3 Pregnancy Proteins/metabolism Protein Transport Sequence Alignment Vesicular Transport Proteins rho GTP-Binding Proteins/chemistry,genetics,metabolism
Chemicals
DNA-Binding Proteins Intracellular Signaling Peptides and Proteins PLIN3 protein, human Perilipin-3 Pregnancy Proteins Vesicular Transport Proteins Adenosine Triphosphate RhoBTB3 protein, human rho GTP-Binding Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Espinosa Eric J
Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305-5307, USA.
Calero Monica
Sridevi Khambhampaty
Pfeffer Suzanne R
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
1097-4172
DOI
10.1016/j.cell.2009.03.043
Published
2009-05-29
Pages
938-48
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC2801561
Subset
IM
Grants
NIDDK NIH HHS · R01 DK037332 · United States
NIDDK NIH HHS · R37 DK037332 · United States
NIDDK NIH HHS · R37 DK037332-23 · United States
NIDDK NIH HHS · R37-DK37332 · United States
External Links
PubMed source DOI: 10.1016/j.cell.2009.03.043 PMC Full Text View journal details
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